PDF(Pubmed)
Abstract：
The domestication process of the common bean gave rise to six different races which come from the two ancestral genetic pools, the Mesoamerican (Durango, Jalisco, and Mesoamerica races) and the Andean (New Granada, Peru, and Chile races). In this study, a collection of 281 common bean landraces from Chile was analyzed using a 12K-SNP microarray. Additionally, 401 accessions representing the rest of the five common bean races were analyzed. A total of 2543 SNPs allowed us to differentiate a genetic group of 165 accessions that corresponds to the race Chile, 90 of which were classified as pure accessions, such as the bean types \'Tórtola\', \'Sapito\', \'Coscorrón\', and \'Frutilla\'. Our genetic analysis indicates that the race Chile has a close relationship with accessions from Argentina, suggesting that nomadic ancestral peoples introduced the bean seed to Chile. Previous archaeological and genetic studies support this hypothesis. Additionally, the low genetic diversity (π = 0.053; uHe = 0.53) and the negative value of Tajima\' D (D = -1.371) indicate that the race Chile suffered a bottleneck and a selective sweep after its introduction, supporting the hypothesis that a small group of Argentine bean genotypes led to the race Chile. A total of 235 genes were identified within haplotype blocks detected exclusively in the race Chile, most of them involved in signal transduction, supporting the hypothesis that intracellular signaling pathways play a fundamental role in the adaptation of organisms to changes in the environment. To date, our findings are the most complete investigation associated with the origin of the race Chile of common bean.
摘要：
普通豆的驯化过程产生了六个不同的种族，它们来自两个祖先的遗传库，中美洲(杜兰戈，哈利斯科州,和中美洲种族）和安第斯（新格拉纳达，秘鲁,和智利种族）。在这项研究中,使用12K-SNP微阵列分析了来自智利的281种常见豆类地方品种的集合。此外,分析了代表五个常见豆类品种中其余品种的401种。总共2543个SNP使我们能够区分165个与智利种族相对应的遗传组，其中90个被归类为纯加入，例如bean类型\'Tórtola\',\'Sapito\',\'Cosorrón\',和\'Fratilla\'。我们的遗传分析表明，智利种族与阿根廷的加入有着密切的关系，表明游牧祖先将豆子种子引入智利。先前的考古和遗传研究支持这一假设。此外,低遗传多样性（π=0.053；uHe=0.53）和Tajima\'D的负值（D=-1.371）表明智利种族在引入后遭受了瓶颈和选择性扫荡，支持一小群阿根廷豆基因型导致智利种族的假设。在仅在智利种族中检测到的单倍型区块中总共鉴定出235个基因，它们中的大多数参与信号转导，支持以下假设：细胞内信号通路在生物体适应环境变化中起着基本作用。迄今为止,我们的发现是与普通豆智利种族起源相关的最完整的调查。
