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Abstract：
Tetraselmis chuii is an EFSA-approved novel food and dietary supplement with increasing use in nutraceutical production worldwide. This study investigated the neuroprotective potential of bioactive compounds extracted from T. chuii using green biobased solvents (ethyl acetate, AcOEt, and cyclopentyl methyl ether, CPME) under pressurized liquid extraction (PLE) conditions and supercritical fluid extraction (SFE). Response surface optimization was used to study the effect of temperature and solvent composition on the neuroprotective properties of the PLE extracts, including anticholinergic activity, reactive oxygen/nitrogen species (ROS/RNS) scavenging capacity, and anti-inflammatory activity. Optimized extraction conditions of 40 °C and 34.9% AcOEt in CPME resulted in extracts with high anticholinergic and ROS/RNS scavenging capacity, while operation at 180 °C and 54.1% AcOEt in CPME yielded extracts with potent anti-inflammatory properties using only 20 min. Chemical characterization revealed the presence of carotenoids (neoxanthin, violaxanthin, zeaxanthin, α- and β-carotene) known for their anti-cholinesterase, antioxidant, and anti-inflammatory potential. The extracts also exhibited high levels of omega-3 polyunsaturated fatty acids (PUFAs) with a favorable ω-3/ω-6 ratio (>7), contributing to their neuroprotective and anti-inflammatory effects. Furthermore, the extracts were found to be safe to use, as cytotoxicity assays showed no observed toxicity in HK-2 and THP-1 cell lines at or below a concentration of 40 μg mL-1. These results highlight the neuroprotective potential of Tetraselmis chuii extracts, making them valuable in the field of nutraceutical production and emphasize the interest of studying new green solvents as alternatives to conventional toxic solvents.
摘要：
Tetraselmischuii是EFSA批准的新型食品和膳食补充剂，在全球营养保健品生产中的使用越来越多。本研究调查了使用绿色生物基溶剂（乙酸乙酯，AcOEt,和环戊基甲基醚，CPME）在加压液体萃取（PLE）和超临界流体萃取（SFE）条件下。响应面优化用于研究温度和溶剂组成对PLE提取物神经保护性能的影响。包括抗胆碱能活性，活性氧/氮（ROS/RNS）清除能力，和抗炎活性。在CPME中40°C和34.9％AcOEt的优化提取条件导致提取物具有高抗胆碱能和ROS/RNS清除能力，在180°C和CPME中的54.1％AcOEt下手术仅使用20分钟就产生了具有有效抗炎特性的提取物。化学表征显示存在类胡萝卜素（新黄质，紫黄质,玉米黄质,α-和β-胡萝卜素）以其抗胆碱酯酶而闻名，抗氧化剂，和抗炎潜力。提取物还表现出高水平的ω-3多不饱和脂肪酸（PUFA），具有良好的ω-3/ω-6比例（>7），有助于它们的神经保护和抗炎作用。此外，提取物被发现使用是安全的，由于细胞毒性分析显示在浓度为40μgmL-1或以下的HK-2和THP-1细胞系中未观察到毒性。这些结果突出了Tetraselmischuii提取物的神经保护潜力，使它们在营养食品生产领域有价值，并强调研究新的绿色溶剂作为常规有毒溶剂的替代品的兴趣。
