PDF(Pubmed)
Abstract：
Lumpy Skin Disease (LSD) is a notifiable viral disease caused by Lumpy Skin Disease virus (LSDV). It is usually associated with high economic losses, including a loss of productivity, infertility, and death. LSDV shares genetic and antigenic similarities with Sheep pox virus (SPV) and Goat pox (GPV) virus. Hence, the LSDV traditional diagnostic tools faced many limitations regarding sensitivity, specificity, and cross-reactivity. Herein, we fabricated a paper-based turn-on fluorescent Molecularly Imprinted Polymer (MIP) sensor for the rapid detection of LSDV. The LSDV-MIPs sensor showed strong fluorescent intensity signal enhancement in response to the presence of the virus within minutes. Our sensor showed a limit of detection of 101 log10 TCID50/mL. Moreover, it showed significantly higher specificity to LSDV relative to other viruses, especially SPV. To our knowledge, this is the first record of a paper-based rapid detection test for LSDV depending on fluorescent turn-on behavior.
摘要：
结块皮肤病(LSD)是由结块皮肤病病毒(LSDV)引起的应报告的病毒性疾病。它通常伴随着很高的经济损失，包括生产力的损失，不孕症,和死亡。LSDV与羊痘病毒（SPV）和羊痘病毒（GPV）具有遗传和抗原相似性。因此，LSDV传统诊断工具在灵敏度方面面临许多限制，特异性，和交叉反应性。在这里，我们制造了一种基于纸的开启荧光分子印迹聚合物（MIP）传感器，用于快速检测LSDV。LSDV-MIP传感器在数分钟内响应于病毒的存在而显示出强的荧光强度信号增强。我们的传感器显示出101log10TCID50/mL的检测限。此外，相对于其他病毒，它对LSDV的特异性明显更高，尤其是SPV。据我们所知,这是对基于荧光开启行为的LSDV的纸质快速检测测试的第一个记录。
