PDF(Pubmed)
Abstract：
Quinoa, known as the \"golden grain\" for its high nutritional value, has polysaccharides as one of its sources of important nutrients. However, the biological functions of quinoa polysaccharides remain understudied. In this study, two crude polysaccharide extracts of quinoa (Q-40 and Q-60) were obtained through sequential precipitation with 40% and 60% ethanol, with purities of 58.29% (HPLC) and 62.15% (HPLC) and a protein content of 8.27% and 9.60%, respectively. Monosaccharide analysis revealed that Q-40 contained glucose (Glc), galacturonic acid (GalA), and arabinose (Ara) in a molar ratio of 0.967:0.027:0.006. Q-60 was composed of xylose (xyl), arabinose (Ara), galactose, and galacturonic acid (GalA) with a molar ratio of 0.889:0.036:0.034:0.020. The average molecular weight of Q-40 ranged from 47,484 to 626,488 Da, while Q-60 showed a range of 10,025 to 47,990 Da. Rheological experiments showed that Q-40 exhibited higher viscosity, while Q-60 demonstrated more elastic properties. Remarkably, Q-60 showed potent antioxidant abilities, with scavenging rates of 98.49% for DPPH and 57.5% for ABTS. Antibacterial experiments using the microdilution method revealed that Q-40 inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA) and Escherichia coli (E. coli), while Q-60 specifically inhibited MRSA. At lower concentrations, both polysaccharides inhibited MDA (MD Anderson Cancer Center) cell proliferation, but at higher concentrations, they promoted proliferation. Similar proliferation-promoting effects were observed in HepG2 cells. The research provides important information in the application of quinoa in the food and functional food industries.
摘要：
藜麦,因其营养价值高而被称为“黄金谷物”，多糖是其重要营养素来源之一。然而,藜麦多糖的生物学功能仍未得到充分研究。在这项研究中,通过用40％和60％乙醇顺序沉淀获得两种藜麦粗多糖提取物（Q-40和Q-60），纯度分别为58.29%（HPLC）和62.15%（HPLC），蛋白质含量分别为8.27%和9.60%，分别。单糖分析表明Q-40含有葡萄糖（Glc），半乳糖醛酸（GalA），和阿拉伯糖(Ara)的摩尔比为0.967:0.027:0.006。Q-60由木糖（xyl）组成，阿拉伯糖(Ara),半乳糖,和摩尔比为0.889:0.036:0.034:0.020的半乳糖醛酸(GalA)。Q-40的平均分子量范围为47,484至626,488Da，而Q-60显示的范围为10,025至47,990Da。流变实验表明，Q-40表现出更高的粘度，而Q-60表现出更多的弹性性能。值得注意的是,Q-60显示出强大的抗氧化能力，DPPH清除率为98.49%，ABTS清除率为57.5%。使用微量稀释法进行的抗菌实验表明，Q-40抑制耐甲氧西林金黄色葡萄球菌（MRSA）和大肠杆菌（E。大肠杆菌)，而Q-60特异性抑制MRSA。在较低的浓度下，两种多糖均抑制MDA（MDAnderson癌症中心）细胞增殖，但是在更高的浓度下，他们促进了扩散。在HepG2细胞中观察到类似的增殖促进作用。该研究为藜麦在食品和功能食品工业中的应用提供了重要信息。
