PDF(Pubmed)
Abstract：
Cotton is a critical crop with massive economic implications worldwide. Verticillium wilt is a soil-borne ailment caused by Verticillium dahliae, which harms the growth and development of cotton. Therefore, investigating the genes associated with resistance to verticillium wilt is of particular significance. In this study, we identified the GhIQD1 gene through transcriptome analysis and experimentally characterized the role of the GhIQD1 gene in cotton against V. dahliae. The findings indicated that GhIQD1 acts as a calmodulin-binding protein. The expression of GhIQD1 was the highest in stems, and the expression level increased significantly following infection with V. dahliae. The expression in resistant cotton varieties was higher than in susceptible cotton varieties. Through overexpression of the GhIQD1 gene in tobacco, these transgenic plants exhibited improved resistance to V. dahliae. In contrast, by silencing the GhIQD1 gene in cotton through VIGS, the resistance to V. dahliae was reduced. Following inoculation, the leaves yellowed, and the disease index was higher. Transcriptome analysis of transgenic tobacco 72 h after inoculation indicated that overexpression of GhIQD1 increased the enrichment of the calmodulin pathway and stimulated the production of plant hormones alongside secondary metabolites. Consequently, we investigated the relationship between the GhIQD1 gene and plant disease-resistant hormones SA, JA, and ABA. In summary, this study uncovered the mechanism by which GhIQD1 conferred resistance to V. dahliae in cotton through positive regulation of JA and ABA, providing crucial information for further research on the adaptation of plants to pathogen invasion.
摘要：
棉花是在全球范围内具有巨大经济影响的关键作物。黄萎病是由大丽花黄萎病引起的土壤传播疾病，危害棉花的生长发育。因此,研究与黄萎病抗性相关的基因具有特别重要的意义。在这项研究中,我们通过转录组分析鉴定了GhIQD1基因，并通过实验表征了GhIQD1基因在棉花抗大丽花弧菌中的作用。研究结果表明，GhIQD1充当钙调蛋白结合蛋白。GhIQD1在茎中的表达量最高，表达水平在大丽花弧菌感染后显著增加。抗性棉品种中的表达高于易感棉品种。通过在烟草中过度表达GhIQD1基因,这些转基因植物对大丽花弧菌表现出改进的抗性。相比之下,通过VIGS沉默棉花中的GhIQD1基因，对V.dahliae的抗性降低。接种后，叶子泛黄，疾病指数较高。接种后72小时对转基因烟草的转录组分析表明，GhIQD1的过表达增加了钙调蛋白途径的富集，并刺激了植物激素和次生代谢产物的产生。因此,我们调查了GhIQD1基因与植物抗病激素SA之间的关系，JA,和ABA。总之,这项研究揭示了GhIQD1通过对JA和ABA的正向调节赋予棉花大丽花抗性的机制，为进一步研究植物对病原体入侵的适应性提供了重要信息。
