PDF(Pubmed)
Abstract：
Sfela is a white brined Greek cheese of protected designation of origin (PDO) produced in the Peloponnese region from ovine, caprine milk, or a mixture of the two. Despite the PDO status of Sfela, very few studies have addressed its properties, including its microbiology. For this reason, we decided to investigate the microbiome of two PDO industrial Sfela cheese samples along with two non-PDO variants, namely Sfela touloumotiri and Xerosfeli. Matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS), 16S rDNA amplicon sequencing and shotgun metagenomics analysis were used to identify the microbiome of these traditional cheeses. Cultured-based analysis showed that the most frequent species that could be isolated from Sfela cheese were Enterococcus faecium, Lactiplantibacillus plantarum, Levilactobacillus brevis, Pediococcus pentosaceus and Streptococcus thermophilus. Shotgun analysis suggested that in industrial Sfela 1, Str. thermophilus dominated, while industrial Sfela 2 contained high levels of Lactococcus lactis. The two artisanal samples, Sfela touloumotiri and Xerosfeli, were dominated by Tetragenococcus halophilus and Str. thermophilus, respectively. Debaryomyces hansenii was the only yeast species with abundance > 1% present exclusively in the Sfela touloumotiri sample. Identifying additional yeast species in the shotgun data was challenging, possibly due to their low abundance. Sfela cheese appears to contain a rather complex microbial ecosystem and thus needs to be further studied and understood. This might be crucial for improving and standardizing both its production and safety measures.
摘要：
Sfela是在伯罗奔尼撒地区从绵羊生产的受保护原产地（PDO）的白色盐水希腊奶酪，山羊奶，或者两者的混合物。尽管Sfela有PDO身份,很少有研究讨论它的性质，包括它的微生物学。出于这个原因,我们决定调查两个PDO工业Sfela奶酪样品以及两个非PDO变体的微生物组，即Sfelatouloumotiri和Xerosfeli。基质辅助激光解吸电离飞行时间质谱(MALDI-TOFMS),使用16SrDNA扩增子测序和鸟枪宏基因组学分析来鉴定这些传统奶酪的微生物组。基于培养的分析表明，可以从Sfela奶酪中分离出的最常见的物种是屎肠球菌，植物乳杆菌，短小左半芽孢杆菌,戊糖片球菌和嗜热链球菌。猎枪分析表明，在工业Sfela1中，Str。嗜热动物占主导地位，而工业Sfela2含有高水平的乳酸乳球菌。两个手工样品，Sfelatouloumotiri和Xerosfeli,以嗜盐四球菌和Str为主。嗜热动物,分别。汉氏酵母是Sfelatouloumotiri样品中唯一丰度>1％的酵母物种。在shot弹枪数据中识别其他酵母物种具有挑战性，可能是由于它们的丰度低。Sfela奶酪似乎含有相当复杂的微生物生态系统，因此需要进一步研究和理解。这对于改善和标准化其生产和安全措施可能至关重要。
