Abstract：
The pathophysiology of Primary Open Angle Glaucoma (POAG) remains poorly understood. Through proteomic analysis of aqueous humour (AH) from POAG patients, we aim to identify changes in protein composition of these samples compared to control samples. High resolution mass spectrometry-based TMT6plex quantitative proteomics analysis is performed on AH samples collected from POAG patients, and compared against a control group of patients with cataracts. Data are available via ProteomeXchange with identifier PXD033153. 1589 proteins were quantified from the aqueous samples using Proteome Discoverer version 2.2 software. Among these proteins, 210 were identified as unique master proteins. The proteins which were up or down-regulated by ±3 fold-change were considered significant. Human neuroblastoma full-length cDNA clone CS0DD006YL02 was significantly upregulated in patients with severe POAG on >2 medications, while actin, cytoplasmic 1, V2-7 protein (fragment), immunoglobulin-like polypeptide 1 and phosphatidylethanolamine-binding protein 4 were only present in these patients with severe POAG on >2 medications. Beta-crystallin B1 and B2, Gamma-crystallin C, D and S were significantly downregulated in the severe POAG ≤2 glaucoma medications group. Beta-crystallin B2, Gamma-crystallin D and GCT-A9 light chain variable region (fragment) were significantly downregulated in the non-severe POAG group. Actin, cytoplasmic 1 was significantly upregulated in subjects with severe POAG who required more than 2 glaucoma medications. Crystallins (Beta-crystallin B1 and B2, Gamma-crystallin C, D and S) were significantly downregulated in subjects with severe POAG who required less than 2 glaucoma medications.
摘要：
原发性开角型青光眼（POAG）的病理生理学仍然知之甚少。通过对POAG患者房水（AH）的蛋白质组学分析，我们的目的是确定这些样品与对照样品相比蛋白质组成的变化。基于高分辨率质谱的TMT6plex定量蛋白质组学分析是对从POAG患者收集的AH样品进行的，并与对照组的白内障患者进行比较。数据可通过具有标识符PXD033153的ProteomeXchange获得。使用ProteomeDiscoverer版本2.2软件从水性样品定量1589种蛋白质。在这些蛋白质中，210被鉴定为独特的主蛋白。被上调或下调±3倍变化的蛋白质被认为是显著的。人神经母细胞瘤全长cDNA克隆CS0DD006YL02在>2种药物治疗的重度POAG患者中显著上调，而肌动蛋白，细胞质1，V2-7蛋白（片段），免疫球蛋白样多肽1和磷脂酰乙醇胺结合蛋白4仅存在于这些使用>2种药物的重度POAG患者中。β-晶状体蛋白B1和B2，γ-晶状体蛋白C，重度POAG≤2次青光眼药物组D和S显著下调。β-晶状体蛋白B2、γ-晶状体蛋白D和GCT-A9轻链可变区(片段)在非重度POAG组中显著下调。肌动蛋白,在需要2种以上青光眼药物的重度POAG患者中，细胞质1显着上调。晶体蛋白（β-晶体蛋白B1和B2，γ-晶体蛋白C，在需要少于2种青光眼药物的重度POAG受试者中，D和S）显着下调。
