Abstract：
BACKGROUND: One of the most recent hormones to be identified and isolated is irisin, extracted from mouse skeletal muscle in 2012. Irisin has been proven to alter blood pressure, which has an impact on blood vessels, enhance endothelial functions, and prevent injury to endothelial cells. The current study aimed to study the effect of irisin on the ultrastructure of the rat thoracic aorta using the transmission electron microscope (TEM).
METHODS: Twenty female rats were recruited for this study and divided into a control group (non-injected), and four experimental groups (injected groups) each consisting of 4 rats. The experimental groups were injected intraperitoneally with different doses of irisin (250ng/mL, 500ng/mL, 1000ng/mL, and 2000ng/mL) twice a week for 4weeks. Then, the descending thoracic aorta of all experimental rats were resected and proceeded with imaging.
RESULTS: The results of this study showed a change in the thickness of the tunica intima, internal elastic lamina, elastic lamellae, and external elastic lamina concerning increasing injected irisin concentration. While there was a significant increase in the thickness of tunica media (P<0.0001) and smooth muscle cells (P<0.05). Also, the results showed a significant increase in the number of elastic lamellae in the tunica media (P<0.0001).
CONCLUSIONS: Irisin had a major impact on the elasticity of the rat thoracic aorta wall, suggesting that it influences the growth factors of the wall and activates smooth muscle cells in addition to endothelial cells.
METHODS: Twenty female rats were recruited for this study and divided into a control group (non-injected), and four experimental groups (injected groups) each consisting of 4 rats. The experimental groups were injected intraperitoneally with different doses of irisin (250ng/mL, 500ng/mL, 1000ng/mL, and 2000ng/mL) twice a week for 4weeks. Then, the descending thoracic aorta of all experimental rats were resected and proceeded with imaging.
RESULTS: The results of this study showed a change in the thickness of the tunica intima, internal elastic lamina, elastic lamellae, and external elastic lamina concerning increasing injected irisin concentration. While there was a significant increase in the thickness of tunica media (P<0.0001) and smooth muscle cells (P<0.05). Also, the results showed a significant increase in the number of elastic lamellae in the tunica media (P<0.0001).
CONCLUSIONS: Irisin had a major impact on the elasticity of the rat thoracic aorta wall, suggesting that it influences the growth factors of the wall and activates smooth muscle cells in addition to endothelial cells.
摘要：
背景：最近被鉴定和分离的激素之一是irisin，2012年从小鼠骨骼肌中提取。Irisin已经被证明可以改变血压,对血管有影响，增强内皮功能，防止内皮细胞损伤.本研究旨在利用透射电镜（TEM）研究irisin对大鼠胸主动脉超微结构的影响。
方法：本研究招募20只雌性大鼠，分为对照组（未注射），和四个实验组(注射组),每组由4只大鼠组成。实验组腹腔注射不同剂量的艾瑞辛(250ng/mL,500ng/mL,1000ng/mL,和2000ng/mL）每周两次，共4周。然后，所有实验大鼠的胸主动脉均被切除并进行成像。
结果：这项研究的结果显示内膜厚度的变化，内部弹性薄层，弹性薄片，和关于增加注射irisin浓度的外部弹性层。而中膜厚度（P<0.0001）和平滑肌细胞厚度（P<0.05）显着增加。此外，结果表明，膜介质中弹性片层的数量显着增加（P<0.0001）。
结论：Irisin对大鼠胸主动脉壁的弹性有重大影响，这表明它影响壁的生长因子并激活除内皮细胞外的平滑肌细胞。
方法：本研究招募20只雌性大鼠，分为对照组（未注射），和四个实验组(注射组),每组由4只大鼠组成。实验组腹腔注射不同剂量的艾瑞辛(250ng/mL,500ng/mL,1000ng/mL,和2000ng/mL）每周两次，共4周。然后，所有实验大鼠的胸主动脉均被切除并进行成像。
结果：这项研究的结果显示内膜厚度的变化，内部弹性薄层，弹性薄片，和关于增加注射irisin浓度的外部弹性层。而中膜厚度（P<0.0001）和平滑肌细胞厚度（P<0.05）显着增加。此外，结果表明，膜介质中弹性片层的数量显着增加（P<0.0001）。
结论：Irisin对大鼠胸主动脉壁的弹性有重大影响，这表明它影响壁的生长因子并激活除内皮细胞外的平滑肌细胞。
