Abstract：
We investigated the effects of Lactuca sativa L. extracts (Lactuc) on pentobarbital-induced sleep in mice to elucidate the mechanisms underlying its impact on sleep quality. Mice were randomly assigned to five groups: control, positive control (diazepam 2 mg/kg b.w.), and three groups orally administered with Lactuc (50, 100, and 200 mg/kg b.w.). After 2 weeks of oral administration and intraperitoneal injections, the mice were killed. We found that the Lactuc-administered groups had significantly reduced sleep latency and increased sleep duration compared with the control group. Furthermore, the oral administration of Lactuc induced a significant increase in mRNA expression and protein expression of adenosine A1 receptor in the brains compared with the expressions in the control group. In addition, the Lactuc-administered groups exhibited significantly higher levels of mRNA expressions of GABAA receptors subunits α2, β2, γ1, and, γ2 in the brain tissue. Therefore, we suggest that Lactuc could be used to develop natural products that effectively improve sleep quality and duration.
摘要：
我们研究了LactucasativaL.提取物（Lactic）对戊巴比妥诱导的小鼠睡眠的影响，以阐明其对睡眠质量影响的潜在机制。将小鼠随机分为五组：对照组，阳性对照（地西泮2mg/kgb.w.），和三组口服Lactuc（50、100和200mg/kgb.w.）。口服和腹腔注射2周后，老鼠被杀死了。我们发现，与对照组相比，Lactec给药组的睡眠潜伏期显着减少，睡眠持续时间增加。此外，与对照组相比，口服Lactuc诱导脑中腺苷A1受体的mRNA表达和蛋白表达显着增加。此外,Lactec给药组表现出明显更高水平的GABAA受体亚基α2，β2，γ1和，脑组织中的γ2。因此,我们建议Lactic可用于开发可有效改善睡眠质量和持续时间的天然产品。
