Abstract：
A polyphasic taxonomic study was carried out on strain ES2T, isolated from sediment of a wetland created to remediate acid drainage from a coal mine. The rod-shaped bacterium formed yellow/orange pigmented colonies and produced the pigment flexirubin. The 16S rRNA gene sequence results assigned the strain to Chryseobacterium, with 98.9 and 98.3 % similarity to Chryseobacterium vietnamense and Chryseobacterium cucumeris, respectively. Computation of the average nucleotide identity and digital DNA-DNA hybridization values with the closest phylogenetic neighbours of ES2T revealed genetic differences at the species level, which were further substantiated by differences in several physiological characteristics. The dominant fatty acids of strain ES2T were iso-C15 : 0, iso-C17 : 1 ω9c, iso C17 : 0 3-OH, and iso-C15 : 0 2-OH. The DNA G+C content was 35.5 mol%. The major polar lipid was phosphatidylethanolamine while menaquinone-6 was the only menaquinone found. This bacterium has been previously shown to possess metallophore activity towards rare earth elements, and based on genome sequencing, possesses all required genes for siderophore production/activity, possibly identifying the source of this unique ability. On the basis of the results obtained here, this bacterium is assigned to the genus Chryseobacterium as representing a new species with the name Chryseobacterium metallicongregator sp. nov., type strain ES2T (=NRRL B-65679T=KCTC 102120T).
摘要：
对菌株ES2T进行了多相分类研究，从湿地的沉积物中分离出来，该湿地用于修复煤矿的酸性排水。杆状细菌形成黄色/橙色色素菌落，并产生色素flexirubin。16SrRNA基因序列结果将菌株分配给金黄杆菌，与vietnamense和黄瓜Chryseobacterium的相似性分别为98.9％和98.3％，分别。与ES2T最接近的系统发育邻居的平均核苷酸身份和数字DNA-DNA杂交值的计算揭示了物种水平的遗传差异，几个生理特征的差异进一步证实了这一点。菌株ES2T的主要脂肪酸是异C15：0，异C17：1ω9c，isoC17:03-OH,和iso-C15:02-OH。DNAG+C含量为35.5mol%。主要的极性脂质是磷脂酰乙醇胺，而甲基萘醌-6是唯一发现的甲基萘醌。这种细菌先前已被证明对稀土元素具有金属矿活性，基于基因组测序，拥有铁载体生产/活动所需的所有基因，可能会识别这种独特能力的来源。根据这里获得的结果，该细菌被分配给金杆菌属，代表一个新物种，名称为金杆菌属。11月。,型菌株ES2T(=NRRLB-65679T=KCTC102120T)。
