Abstract：
Aniline (C6H5NH2) is one of the hazardous aromatic amine where an amino group -NH2) is connected to phenyl ring (C6H5). Based on the evaluation of the 96-hour LC50 of aniline, two sublethal concentrations (4.19 mg/l and 8.39 mg/l) were selected for acute exposure tests in freshwater fish Channa punctatus. The liver, gills and kidney of fish being the principal sites of xenobiotic material accumulation, respiration, biotransformation, and excretion are the focus of the present study. Throughout the exposure time, the comet assay revealed increased tail length and tail DNA percentage indicating maximum damage to liver, gills and kidney of treated group after 96 h. After acute exposure, there was a significant (p ≤ 0.05) increase in the enzymatic activity of glutathione-S-transferase (GST) and acetylcholinesterase (AChE), whereas decline in superoxide dismutase (SOD) and catalase (CAT) activity was observed. Meanwhile, levels of malondialdehyde (MDA) increased over the exposure period for both concentrations. After 96 h of exposure, degree of tissue change (DTC) was evaluated in liver, gill and kidney of aniline exposed fish. Additionally, light microscopy revealed multiple abnormalities in liver, gills and kidney of all the treated groups. Significant changes were observed in the levels of biochemical markers viz., glucose, triglyceride, cholesterol, aspartate transaminase, alanine transaminase and urea following a 96-hour exposure to aniline. Studies using ATR-FTIR and transmission electron microscopy (TEM) revealed changes in biomolecules and structural abnormalities in several tissues of the aniline-exposed groups in comparison to the control group respectively.
摘要：
苯胺(C6H5NH2)是其中氨基(-NH2)与苯环(C6H5)连接的危险芳族胺之一。根据对苯胺96小时LC50的评估，选择了两个亚致死浓度（4.19mg/l和8.39mg/l）用于淡水鱼Channapunctatus的急性暴露测试。肝脏，鱼的g和肾脏是异种生物物质积累的主要部位，呼吸,生物转化,和排泄是本研究的重点。在整个曝光时间里，彗星试验显示增加的尾部长度和尾部DNA百分比表明对肝脏的最大损害，治疗组96小时后的ill和肾脏。急性暴露后，谷胱甘肽-S-转移酶（GST）和乙酰胆碱酯酶（AChE）的酶活性显着增加（p≤0.05），而观察到超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性下降。同时,丙二醛（MDA）的水平在两种浓度的暴露期间均增加。暴露96小时后，在肝脏中评估组织变化程度（DTC），苯胺暴露鱼的ill和肾脏。此外,光学显微镜显示肝脏有多个异常，所有治疗组的ill和肾脏。观察到生化标志物水平的显着变化。,葡萄糖，甘油三酯，胆固醇,天冬氨酸转氨酶,丙氨酸转氨酶和尿素暴露于苯胺96小时后。使用ATR-FTIR和透射电子显微镜（TEM）进行的研究显示，与对照组相比，苯胺暴露组的几种组织中生物分子和结构异常的变化。
