Abstract：
The present study investigates the use of dextrins (maltodextrin, β-cyclodextrin, and hydroxypropyl-β-cyclodextrin) to improve the efficiency of the agarose-based gel electromembrane extraction technique for extracting chiral basic drugs (citalopram, hydroxyzine, and cetirizine). Additionally, it examines the enantioselectivity of the extraction process for these drugs. To achieve these, dextrins were incorporated into either the sample solution, the membrane, or the acceptor solution, and then the extraction procedure was performed. Enantiomers were separated and analyzed using a capillary electrophoresis device equipped with a UV detector. The results obtained under the optimal extraction conditions (sample solution pH: 4.0, acceptor solution pH: 2.0, gel membrane pH: 3.0, agarose concentration: 3 % w/v, stirring rate: 1000 rpm, gel thickness: 4.4 mm, extraction voltage: 62.3 V, and extraction time: 32.1 min) indicated that incorporating dextrins into either the sample solution, membrane or the acceptor solution enhances extraction efficiency by 17.3-23.1 %. The most significant increase was observed when hydroxypropyl-β-cyclodextrin was added to the acceptor solution. The findings indicated that the inclusion of hydroxypropyl-β-cyclodextrin in the sample solution resulted in an enantioselective extraction, yielding an enantiomeric excess of 6.42-7.14 %. The proposed method showed a linear range of 5.0-2000 ng/mL for enantiomers of model drugs. The limit of detection and limit of quantification for all enantiomers were found to be < 4.5 ng/mL and <15.0 ng/mL, respectively. Intra- and inter-day RSDs (n = 4) were less than 10.8 %, and the relative errors were less than 3.2 % for all the enantiomers. Finally, the developed method was successfully applied to determine concentrations of enantiomers in a urine sample with relative recoveries of 96.8-99.2 %, indicating good reliability of the developed method.
摘要：
本研究调查了糊精(麦芽糖糊精，β-环糊精,和羟丙基-β-环糊精），以提高基于琼脂糖的凝胶电膜提取技术提取手性碱性药物（西酞普兰，羟嗪,和西替利嗪）。此外,它检查了这些药物的提取过程的对映选择性。为了实现这些，将糊精掺入样品溶液中，膜，或受体溶液，然后进行提取程序。使用配备有UV检测器的毛细管电泳装置分离和分析对映体。在最佳提取条件下获得的结果（样品溶液pH：4.0，受体溶液pH：2.0，凝胶膜pH：3.0，琼脂糖浓度：3％w/v，搅拌速率:1000转/分钟,凝胶厚度：4.4毫米，提取电压：62.3V，和提取时间：32.1分钟）表明将糊精掺入任一样品溶液中，膜或受体溶液将提取效率提高17.3-23.1%。当将羟丙基-β-环糊精添加到受体溶液中时,观察到最显著的增加。结果表明，样品溶液中包含羟丙基-β-环糊精会导致对映选择性萃取，产生对映体过量6.42-7.14%。所提出的方法显示模型药物对映体的线性范围为5.0-2000ng/mL。发现所有对映体的检测限和定量限<4.5ng/mL和<15.0ng/mL，分别。日内和日间RSD(n=4)低于10.8%，所有对映体的相对误差均小于3.2%。最后,该方法成功地应用于尿液样品中对映体的浓度测定，相对回收率为96.8-99.2%，表明所开发方法具有良好的可靠性。
