Abstract：
An HPLC-MS/MS multi-class method for quantitation of 15 different classes of veterinary drug residues (>140 analytes) in milk and poultry feed was developed and validated. Accuracy criteria for routine laboratories were met for the majority of analytes, > 83 % in milk and between 50 and 60 % in chicken feed, with an apparent recovery of 60-140 %. Extraction efficiency criteria were met for >95 % of the analytes for milk and > 80 % for chicken feed. Intermediate precision meets the SANTE criterion of RSD < 20 % for 80-90 % of the analytes in both matrices. For all analytes with an existing MRL in milk, the LOQ was below the related MRL. Twenty-nine samples of commercial milk and chicken feed were analyzed within the interlaboratory comparison. No residues of veterinary drugs were found in the milk samples. However, the feed samples exhibited high levels of nicarbazin, salinomycin, and decoquinate.
摘要：
开发并验证了HPLC-MS/MS多类方法，用于定量牛奶和家禽饲料中15种不同类别的兽药残留（>140分析物）。大多数分析物符合常规实验室的准确性标准，>83%的牛奶和50-60%的鸡饲料，表观回收率为60-140%。对于>95%的分析物,牛奶和>80%的鸡饲料,满足提取效率标准。对于两种基质中的80-90%的分析物,中间精密度满足RSD<20%的SANTE标准。对于牛奶中存在MRL的所有分析物，LOQ低于相关MRL。在实验室间比较中分析了29种商品牛奶和鸡肉饲料样品。牛奶样品中未发现兽药残留。然而,饲料样品显示出高水平的尼卡巴嗪，盐霉素,和去碱。
