Abstract：
This study aimed to evaluate the antioxidant potential of autochthonous Hydnum repandum through LC-MS/MS profiling, total phenolic content (TP), total protein content (TPR), and antioxidant capabilities (DPPH, ABTS, and FRAP assays) across various extracts (CHCl3, acetone, 70% EtOH, 80% MeOH, and hot water). LC-MS/MS analysis revealed a predominant presence of quinic acid in polar solvents (ranging from 531.37 to 676.07 ng/mL), while EtOH and MeOH extracts exhibited elevated total phenolic levels (27.44 ± 0.32 and 28.29 ± 3.62 mg GAE/g d.w., respectively). Impressively, H. repandum showcased remarkable antioxidant properties, as evidenced by its FRAP values (57.29 to 199.96 mg AAE/g d.w.), ABTS values (5.69 to 29.95 mg TE/g d.w.), and IC50 values in the DPPH assay (91.40 to 372.55 μg/mL), which exhibited a strong correlation with TP. Notably, the acetone extract exhibited the most robust antioxidant activity where the highest TPR was observed, suggesting synergism of primary and secondary metabolites.
摘要：
本研究旨在通过LC-MS/MS分析评估自生hynchthumrepandum的抗氧化潜力，总酚含量（TP），总蛋白质含量（TPR），和抗氧化能力(DPPH，ABTS,和FRAP测定）跨各种提取物（CHCl3，丙酮，70%EtOH,80%MeOH,和热水）。LC-MS/MS分析显示，在极性溶剂中主要存在奎尼酸（范围为531.37至676.07ng/mL），而EtOH和MeOH提取物显示出升高的总酚水平（27.44±0.32和28.29±3.62mgGAE/gd.w.，分别)。令人印象深刻的是,H.repandum展示了显着的抗氧化性能，其FRAP值（57.29至199.96mgAAE/gd.w.）证明，ABTS值（5.69至29.95mgTE/gd.w.），和DPPH测定中的IC50值（91.40至372.55μg/mL），与TP有很强的相关性。值得注意的是,丙酮提取物表现出最强大的抗氧化活性，其中观察到最高的TPR，提示初级和次级代谢产物的协同作用。
