Abstract：
BACKGROUND: The intestine is a barrier resisting various stress responses. Intrauterine growth restriction (IUGR) can cause damage to the intestinal barrier via destroying the balance of intestinal epithelial cells\' proliferation and apoptosis. Bacillus subtilis has been reported to regulate intestinal epithelial cells\' proliferation and apoptosis. Thus, the purpose of this study was to determine if B. subtilis could regulate intestinal epithelial cells\' proliferation and apoptosis in intrauterine growth restriction suckling piglets.
RESULTS: Compared with the normal birth weight group, the IUGR group showed greater mean optical density values of Ki-67-positive cells in the ileal crypt (P < 0.05). IUGR resulted in higher ability of proliferation and apoptosis of intestinal epithelial cells, by upregulation of the messenger RNA (mRNA) or proteins expression of leucine rich repeat containing G protein coupled receptor 5, Caspase-3, Caspase-7, β-catenin, cyclinD1, B-cell lymphoma-2 associated agonist of cell death, and BCL2 associated X (P < 0.05), and downregulation of the mRNA or protein expression of B-cell lymphoma-2 and B-cell lymphoma-2-like 1 (P < 0.05). However, B. subtilis supplementation decreased the mRNA or proteins expression of leucine rich repeat containing G protein coupled receptor 5, SPARC related modular calcium binding 2, tumor necrosis factor receptor superfamily member 19, cyclinD1, Caspase-7, β-catenin, B-cell lymphoma-2 associated agonist of cell death, and Caspase-3 (P < 0.05), and increased the mRNA expression of B-cell lymphoma-2 (P < 0.05).
CONCLUSIONS: IUGR led to excessive apoptosis of intestinal epithelial cells, which induced compensatory proliferation. However, B. subtilis treatment prevented intestinal epithelial cells of IUGR suckling piglets from excessive apoptosis. © 2024 Society of Chemical Industry.
RESULTS: Compared with the normal birth weight group, the IUGR group showed greater mean optical density values of Ki-67-positive cells in the ileal crypt (P < 0.05). IUGR resulted in higher ability of proliferation and apoptosis of intestinal epithelial cells, by upregulation of the messenger RNA (mRNA) or proteins expression of leucine rich repeat containing G protein coupled receptor 5, Caspase-3, Caspase-7, β-catenin, cyclinD1, B-cell lymphoma-2 associated agonist of cell death, and BCL2 associated X (P < 0.05), and downregulation of the mRNA or protein expression of B-cell lymphoma-2 and B-cell lymphoma-2-like 1 (P < 0.05). However, B. subtilis supplementation decreased the mRNA or proteins expression of leucine rich repeat containing G protein coupled receptor 5, SPARC related modular calcium binding 2, tumor necrosis factor receptor superfamily member 19, cyclinD1, Caspase-7, β-catenin, B-cell lymphoma-2 associated agonist of cell death, and Caspase-3 (P < 0.05), and increased the mRNA expression of B-cell lymphoma-2 (P < 0.05).
CONCLUSIONS: IUGR led to excessive apoptosis of intestinal epithelial cells, which induced compensatory proliferation. However, B. subtilis treatment prevented intestinal epithelial cells of IUGR suckling piglets from excessive apoptosis. © 2024 Society of Chemical Industry.
摘要：
背景：肠是抵抗各种应激反应的屏障。宫内生长受限（IUGR）可通过破坏肠上皮细胞增殖和凋亡的平衡而引起肠屏障的破坏。已报道枯草芽孢杆菌调节肠上皮细胞的增殖和凋亡。因此，本研究的目的是确定枯草芽孢杆菌是否可以调节宫内生长受限乳猪肠上皮细胞的增殖和凋亡。
结果：与正常出生体重组相比，IUGR组回肠隐窝中Ki-67阳性细胞的平均光密度值较大（P<0.05）。IUGR导致肠上皮细胞增殖和凋亡能力提高,通过上调富含亮氨酸重复序列的G蛋白偶联受体5，Caspase-3，Caspase-7，β-catenin的mRNA或蛋白质表达，cyclinD1，B细胞淋巴瘤-2相关的细胞死亡激动剂，与BCL2相关的X（P<0.05），B细胞淋巴瘤-2和B细胞淋巴瘤-2样1的mRNA或蛋白表达下调(P<0.05)。然而,补充枯草芽孢杆菌降低了富含亮氨酸重复序列的G蛋白偶联受体5，SPARC相关模块钙结合2，肿瘤坏死因子受体超家族成员19，cyclinD1，Caspase-7，β-catenin的mRNA或蛋白质表达，B细胞淋巴瘤-2相关的细胞死亡激动剂，和Caspase-3（P<0.05），B细胞淋巴瘤-2的mRNA表达增加(P<0.05)。
结论：IUGR导致肠上皮细胞过度凋亡，诱导代偿性增殖。然而,枯草芽孢杆菌治疗可防止IUGR乳猪肠上皮细胞过度凋亡。本文受版权保护。保留所有权利。
结果：与正常出生体重组相比，IUGR组回肠隐窝中Ki-67阳性细胞的平均光密度值较大（P<0.05）。IUGR导致肠上皮细胞增殖和凋亡能力提高,通过上调富含亮氨酸重复序列的G蛋白偶联受体5，Caspase-3，Caspase-7，β-catenin的mRNA或蛋白质表达，cyclinD1，B细胞淋巴瘤-2相关的细胞死亡激动剂，与BCL2相关的X（P<0.05），B细胞淋巴瘤-2和B细胞淋巴瘤-2样1的mRNA或蛋白表达下调(P<0.05)。然而,补充枯草芽孢杆菌降低了富含亮氨酸重复序列的G蛋白偶联受体5，SPARC相关模块钙结合2，肿瘤坏死因子受体超家族成员19，cyclinD1，Caspase-7，β-catenin的mRNA或蛋白质表达，B细胞淋巴瘤-2相关的细胞死亡激动剂，和Caspase-3（P<0.05），B细胞淋巴瘤-2的mRNA表达增加(P<0.05)。
结论：IUGR导致肠上皮细胞过度凋亡，诱导代偿性增殖。然而,枯草芽孢杆菌治疗可防止IUGR乳猪肠上皮细胞过度凋亡。本文受版权保护。保留所有权利。
