RESULTS: Compared with the normal birth weight group, the IUGR group showed greater mean optical density values of Ki-67-positive cells in the ileal crypt (P < 0.05). IUGR resulted in higher ability of proliferation and apoptosis of intestinal epithelial cells, by upregulation of the messenger RNA (mRNA) or proteins expression of leucine rich repeat containing G protein coupled receptor 5, Caspase-3, Caspase-7, β-catenin, cyclinD1, B-cell lymphoma-2 associated agonist of cell death, and BCL2 associated X (P < 0.05), and downregulation of the mRNA or protein expression of B-cell lymphoma-2 and B-cell lymphoma-2-like 1 (P < 0.05). However, B. subtilis supplementation decreased the mRNA or proteins expression of leucine rich repeat containing G protein coupled receptor 5, SPARC related modular calcium binding 2, tumor necrosis factor receptor superfamily member 19, cyclinD1, Caspase-7, β-catenin, B-cell lymphoma-2 associated agonist of cell death, and Caspase-3 (P < 0.05), and increased the mRNA expression of B-cell lymphoma-2 (P < 0.05).
CONCLUSIONS: IUGR led to excessive apoptosis of intestinal epithelial cells, which induced compensatory proliferation. However, B. subtilis treatment prevented intestinal epithelial cells of IUGR suckling piglets from excessive apoptosis. © 2024 Society of Chemical Industry.
结果:与正常出生体重组相比,IUGR组回肠隐窝中Ki-67阳性细胞的平均光密度值较大(P<0.05)。IUGR导致肠上皮细胞增殖和凋亡能力提高,通过上调富含亮氨酸重复序列的G蛋白偶联受体5,Caspase-3,Caspase-7,β-catenin的mRNA或蛋白质表达,cyclinD1,B细胞淋巴瘤-2相关的细胞死亡激动剂,与BCL2相关的X(P<0.05),B细胞淋巴瘤-2和B细胞淋巴瘤-2样1的mRNA或蛋白表达下调(P<0.05)。然而,补充枯草芽孢杆菌降低了富含亮氨酸重复序列的G蛋白偶联受体5,SPARC相关模块钙结合2,肿瘤坏死因子受体超家族成员19,cyclinD1,Caspase-7,β-catenin的mRNA或蛋白质表达,B细胞淋巴瘤-2相关的细胞死亡激动剂,和Caspase-3(P<0.05),B细胞淋巴瘤-2的mRNA表达增加(P<0.05)。
结论:IUGR导致肠上皮细胞过度凋亡,诱导代偿性增殖。然而,枯草芽孢杆菌治疗可防止IUGR乳猪肠上皮细胞过度凋亡。本文受版权保护。保留所有权利。