PDF(Pubmed)
Abstract：
Cellular magnetic field effects are assumed to base on coherent singlet-triplet interconversion of radical pairs that are sensitive to applied radiofrequency (RF) and weak magnetic fields (WEMFs), known as radical pair mechanism (RPM). As a leading model, the RPM explains how quantum effects can influence biochemical and cellular signalling. Consequently, radical pairs generate reactive oxygen species (ROS) that link the RPM to redox processes, such as the response to hypoxia and the circadian clock. Therapeutic nuclear magnetic resonance (tNMR) occupies a unique position in the RPM paradigm because of the used frequencies, which are far below the range of 0.1-100 MHz postulated for the RPM to occur. Nonetheless, tNMR was shown to induce RPM like effects, such as increased extracellular H2O2 levels and altered cellular bioenergetics. In this study we compared the impact of tNMR and intermittent hypoxia on the circadian clock, as well as the role of superoxide in tNMR induced ROS partitioning. We show that both, tNMR and intermittent hypoxia, exert on/off effects on cellular clocks that are dependent on the time of application (day versus night). In addition, our data provide further evidence that superoxide plays a central role in magnetic signal transduction. tNMR used in combination with scavengers, such as Vitamin C, led to strong ROS product redistributions. This discovery might represent the first indication of radical triads in biological systems.
摘要：
假定细胞磁场效应基于对施加的射频（RF）和弱磁场（WEMF）敏感的自由基对的相干单重态-三重态相互转换，称为自由基对机制（RPM）。作为一个领先的模型，RPM解释了量子效应如何影响生化和细胞信号。因此,自由基对产生活性氧（ROS），将RPM与氧化还原过程联系起来，例如对缺氧的反应和生物钟。由于使用的频率，治疗性核磁共振（tNMR）在RPM范例中占据独特的位置，远低于假定发生RPM的0.1-100MHz的范围。尽管如此，tNMR显示诱导RPM样效应，例如细胞外H2O2水平增加和细胞生物能量学改变。在这项研究中，我们比较了tNMR和间歇性缺氧对昼夜节律的影响，以及超氧化物在tNMR诱导的ROS分配中的作用。我们证明了两者，tNMR和间歇性缺氧，对依赖于应用时间(白天与夜晚)的细胞时钟施加开/关效应。此外,我们的数据进一步证明了超氧化物在磁信号转导中起着核心作用.tNMR与清除剂结合使用，比如维生素C,导致了强劲的ROS产品再分配。这一发现可能代表了生物系统中激进三合会的第一个迹象。
