PDF(Pubmed)
Abstract：
Pulchinenoside B4, a natural saponin monomer from the Pulsatilla plant, plays an important role as an immunomodulator in the treatment of acute inflammation. Oral ulcer (OU) is a common ulcerative injury disease that occurs in the oral mucosa, including mucosal ulceration and abnormalities of lips and tongue. A close correlation exists between gut microbiota and circulating metabolites in patients with OU. However, the correlation between gut microbiota and serum metabolomics is not clear. Therefore, this study aimed to explore the changes in gut microbiota and metabolites in OU. The 16S ribosomal RNA (16S rRNA) gene sequencing was used to detect the changes in the composition of gut microbiota in OU rat model. Moreover, the endogenous small metabolites were explored by collecting the non-targeted serum metabolomics data. A total of 34 OU-related biomarkers were identified, mainly related to fatty acid metabolism and inflammatory pathways. The administration of B4 effectively reduced the occurrence of OU and restored the levels of multiple endogenous biomarkers and key gut microbial species to the normal level. This study demonstrated that the gut microbiota and metabolites were altered in the OU rat model, which were significantly restored to the normal level by B4, thereby showing good application prospects in the treatment of OU. KEY POINTS: • The first investigating the correlation between OU and gut microbiota. • A close correlation between metabolites and gut microbiota in OU disease was successfully identified. • Pulchinenoside B4 ameliorates oral ulcers in rats by modulating gut microbiota and metabolites.
摘要：
白头翁皂苷B4是一种来自白头翁属植物的天然皂苷单体，作为免疫调节剂在急性炎症的治疗中起着重要作用。口腔溃疡(OU)是一种常见的发生于口腔黏膜的溃疡性损伤性疾病。包括粘膜溃疡和嘴唇和舌头的异常。OU患者的肠道菌群与循环代谢产物之间存在密切的相关性。然而,肠道菌群与血清代谢组学之间的相关性尚不清楚。因此,本研究旨在探讨OU肠道菌群和代谢产物的变化。采用16S核糖体RNA(16SrRNA)基因测序法检测OU大鼠模型肠道菌群组成的变化。此外，通过收集非靶向血清代谢组学数据对内源性小代谢产物进行了研究.总共确定了34个OU相关的生物标志物，主要与脂肪酸代谢和炎症通路有关。B4的给药有效地减少了OU的发生，并将多种内源性生物标志物和关键肠道微生物物种的水平恢复到正常水平。这项研究表明，肠道菌群和代谢产物在OU大鼠模型中发生了改变，B4显著恢复至正常水平,在OU治疗中显示出良好的应用前景。关键点：•首次调查OU与肠道微生物群之间的相关性。•成功鉴定了OU疾病中代谢物与肠道微生物群之间的紧密相关性。•PulchinenosideB4通过调节肠道微生物群和代谢物改善大鼠口腔溃疡。
