Abstract：
It is crucial to clarify the stability of Kombucha in the manufacture and storage stages due to the extensive study on the fermented products of Kombucha and the increase in the use of bacterial cellulose (BC). This study aimed to evaluate the stability of Kombucha in different manufacturing and storage temperatures within a certain time period. The stability of microorganisms and BC in Kombucha was investigated through regular replacement with the tea media at 28 and 25°C for manufacture, and the storage temperature of Kombucha was at 25, 4, and -20°C. Morphological observations of the BC in Kombucha ended at 28 and 25°C for manufacture and storage were performed using atomic force microscopy (AFM) before inoculation. The viable cell counts and AFM results showed that the stability of Kombucha during manufacture was better at 28°C than at 25°C, with higher microbial viability and BC productivity in the former at the time of manufacture, whereas 25°C was more favorable for the stability of Kombucha during storage. At the same temperature of 25°C, the manufacturing practice improved the microbial viability and BC stability compared with storage; the pH value of Kombucha was lower, and the dry weight of BC was higher during storage compared with manufacture. The maximum BC water holding capacity (97.16%) was maintained by storage at 4°C on day 63, and the maximum BC swelling rate (56.92%) was observed after storage at -20°C on day 7. The research was conducted to provide reference information for applying Kombucha and its BC in food and development in other industries.
摘要：
由于对红茶菌发酵产物的广泛研究和细菌纤维素（BC）使用的增加，澄清红茶菌在生产和储存阶段的稳定性至关重要。本研究旨在评估康普茶在不同制造和储存温度下一定时间内的稳定性。通过在28和25°C下定期更换茶介质进行生产，研究了红茶菌中微生物和BC的稳定性，红茶菌的储存温度为25、4和-20℃。在接种前，使用原子力显微镜（AFM）对红茶菌中的BC进行形态观察，以在28和25°C下生产和储存。活细胞计数和AFM结果表明，红茶菌在制造过程中的稳定性在28°C下比在25°C下好。前者在制造时具有更高的微生物活力和BC生产率，而25°C更有利于红茶菌在储存过程中的稳定性。在25°C的相同温度下，生产实践与储存相比，提高了微生物活力和BC稳定性；红茶菌的pH值更低，与制造相比，储存期间BC的干重较高。通过在第63天在4°C下储存保持最大BC持水量（97.16％），在第7天在-20°C下储存后观察到最大BC溶胀率（56.92％）。本研究旨在为康普茶及其BC在食品中的应用和其他行业的发展提供参考信息。
