PDF(Pubmed)
Abstract：
BACKGROUND: In Eukaryotes, inositol polyphosphates (InsPs) represent a large family of secondary messengers and play crucial roes in various cellular processes. InsPs are synthesized through a series of pohophorylation reactions catalyzed by various InsP kinases in a sequential manner. Inositol 1,4,5-trisphosphate 3-kinase (IP3 3-kinase/IP3K), one member of InsP kinase, plays important regulation roles in InsPs metabolism by specifically phosphorylating inositol 1,4,5-trisphosphate (IP3) to inositol 1,3,4,5-tetrakisphosphate (IP4) in animal cells. IP3Ks were widespread in fungi, plants and animals. However, its evolutionary history and patterns have not been examined systematically.
RESULTS: A total of 104 and 31 IP3K orthologues were identified across 57 plant genomes and 13 animal genomes, respectively. Phylogenetic analyses indicate that IP3K originated in the common ancestor before the divergence of fungi, plants and animals. In most plants and animals, IP3K maintained low-copy numbers suggesting functional conservation during plant and animal evolution. In Brassicaceae and vertebrate, IP3K underwent one and two duplication events, respectively, resulting in multiple gene copies. Whole-genome duplication (WGD) was the main mechanism for IP3K duplications, and the IP3K duplicates have experienced functional divergence. Finally, a hypothetical evolutionary model for the IP3K proteins is proposed based on phylogenetic theory.
CONCLUSIONS: Our study reveals the evolutionary history of IP3K proteins and guides the future functions of animal, plant, and fungal IP3K proteins.
RESULTS: A total of 104 and 31 IP3K orthologues were identified across 57 plant genomes and 13 animal genomes, respectively. Phylogenetic analyses indicate that IP3K originated in the common ancestor before the divergence of fungi, plants and animals. In most plants and animals, IP3K maintained low-copy numbers suggesting functional conservation during plant and animal evolution. In Brassicaceae and vertebrate, IP3K underwent one and two duplication events, respectively, resulting in multiple gene copies. Whole-genome duplication (WGD) was the main mechanism for IP3K duplications, and the IP3K duplicates have experienced functional divergence. Finally, a hypothetical evolutionary model for the IP3K proteins is proposed based on phylogenetic theory.
CONCLUSIONS: Our study reveals the evolutionary history of IP3K proteins and guides the future functions of animal, plant, and fungal IP3K proteins.
摘要：
背景：在真核生物中，肌醇多磷酸盐（InsPs）代表了第二信使的大家庭，并在各种细胞过程中起着至关重要的作用。InsP是通过一系列由各种InsP激酶以顺序方式催化的泊霍化反应合成的。肌醇1,4,5-三磷酸3-激酶（IP33-激酶/IP3K），InsP激酶的一个成员，通过将动物细胞中的肌醇1,4,5-三磷酸肌醇（IP3）特异性磷酸化为肌醇1,3,4,5-四磷酸肌醇（IP4），在InsPs代谢中起着重要的调节作用。IP3K在真菌中广泛存在，植物和动物。然而,它的进化历史和模式还没有被系统地研究。
结果：在57个植物基因组和13个动物基因组中，共鉴定出104个和31个IP3K直系同源物。分别。系统发育分析表明，IP3K起源于真菌分化之前的共同祖先，植物和动物。在大多数植物和动物中，IP3K保持低拷贝数，表明植物和动物进化过程中的功能保护。在十字花科和脊椎动物中，IP3K经历了一次和两次重复事件，分别,导致多个基因拷贝。全基因组复制（WGD）是IP3K复制的主要机制，和IP3K重复经历了功能分歧。最后,基于系统发育理论，提出了IP3K蛋白的假设进化模型。
结论：我们的研究揭示了IP3K蛋白的进化史，并指导动物的未来功能，植物,和真菌IP3K蛋白。
结果：在57个植物基因组和13个动物基因组中，共鉴定出104个和31个IP3K直系同源物。分别。系统发育分析表明，IP3K起源于真菌分化之前的共同祖先，植物和动物。在大多数植物和动物中，IP3K保持低拷贝数，表明植物和动物进化过程中的功能保护。在十字花科和脊椎动物中，IP3K经历了一次和两次重复事件，分别,导致多个基因拷贝。全基因组复制（WGD）是IP3K复制的主要机制，和IP3K重复经历了功能分歧。最后,基于系统发育理论，提出了IP3K蛋白的假设进化模型。
结论：我们的研究揭示了IP3K蛋白的进化史，并指导动物的未来功能，植物,和真菌IP3K蛋白。
