Abstract：
OBJECTIVE: Madecassoside (MA) is a triterpene derived from Centella asiatica that has been recognized for its antioxidant and anti-inflammatory properties in various disease models. However, its direct impact on cultured white adipocytes and the underlying mechanisms, mainly through gene knockdown, have not been thoroughly explored.
METHODS: Western blot analysis was utilized to assess the expression levels of various proteins, while oil red O staining was used to measure lipid deposition. The adipocyte shapes were confirmed using H&E staining.
RESULTS: MA treatment enhanced browning and lipolysis in 3T3-L1 adipocytes and adipose tissue from experimental mice while suppressing lipogenesis. Furthermore, MA treatment increased the expression of PPARα and FGF21 in 3T3-L1 adipocytes as well as the secretion of FGF21 into the culture medium. Knockdown of PPARα or FGF21 using siRNA diminished the effects of MA on lipid metabolism in cultured adipocytes.
CONCLUSIONS: These findings demonstrate that MA promotes thermogenic browning and lipolysis while inhibiting adipocyte lipogenesis, thus showing the potential for attenuating obesity. The study suggested that MA could be a viable therapeutic approach for treating obesity.
METHODS: Western blot analysis was utilized to assess the expression levels of various proteins, while oil red O staining was used to measure lipid deposition. The adipocyte shapes were confirmed using H&E staining.
RESULTS: MA treatment enhanced browning and lipolysis in 3T3-L1 adipocytes and adipose tissue from experimental mice while suppressing lipogenesis. Furthermore, MA treatment increased the expression of PPARα and FGF21 in 3T3-L1 adipocytes as well as the secretion of FGF21 into the culture medium. Knockdown of PPARα or FGF21 using siRNA diminished the effects of MA on lipid metabolism in cultured adipocytes.
CONCLUSIONS: These findings demonstrate that MA promotes thermogenic browning and lipolysis while inhibiting adipocyte lipogenesis, thus showing the potential for attenuating obesity. The study suggested that MA could be a viable therapeutic approach for treating obesity.
摘要：
目的：羟基积雪草苷（MA）是一种源自积雪草的三萜，已在各种疾病模型中被认为具有抗氧化和抗炎特性。然而,它对培养的白色脂肪细胞的直接影响和潜在的机制，主要是通过基因敲除,还没有被彻底探索。
方法：蛋白质印迹分析用于评估各种蛋白质的表达水平，油红O染色用于测量脂质沉积。使用H&E染色确认脂肪细胞形状。
结果：MA处理增强了来自实验小鼠的3T3-L1脂肪细胞和脂肪组织的褐变和脂解，同时抑制了脂肪生成。此外，MA处理增加了3T3-L1脂肪细胞中PPARα和FGF21的表达以及FGF21向培养基中的分泌。使用siRNA敲除PPARα或FGF21减少了MA对培养的脂肪细胞中脂质代谢的影响。
结论：这些发现表明MA促进热褐变和脂解，同时抑制脂肪细胞脂肪生成，从而显示出减轻肥胖的潜力。研究表明，MA可能是治疗肥胖症的可行治疗方法。
方法：蛋白质印迹分析用于评估各种蛋白质的表达水平，油红O染色用于测量脂质沉积。使用H&E染色确认脂肪细胞形状。
结果：MA处理增强了来自实验小鼠的3T3-L1脂肪细胞和脂肪组织的褐变和脂解，同时抑制了脂肪生成。此外，MA处理增加了3T3-L1脂肪细胞中PPARα和FGF21的表达以及FGF21向培养基中的分泌。使用siRNA敲除PPARα或FGF21减少了MA对培养的脂肪细胞中脂质代谢的影响。
结论：这些发现表明MA促进热褐变和脂解，同时抑制脂肪细胞脂肪生成，从而显示出减轻肥胖的潜力。研究表明，MA可能是治疗肥胖症的可行治疗方法。
