{Reference Type}: Journal Article
{Title}: Madecassoside modulates lipid metabolism in visceral adipocytes: exploring the browning, lipolysis, and lipogenesis mechanisms for potential obesity treatment.
{Author}: Cho W;Hong M;Mobarak EH;Birdal O;Lim MC;Jung MS;Hong SA;Jeong JH;Jung TW;
{Journal}: J Pharm Pharmacol
{Volume}: 76
{Issue}: 7
{Year}: 2024 Jul 5
{Factor}: 4.81
{DOI}: 10.1093/jpp/rgae042
{Abstract}: <B>OBJECTIVE: </B>Madecassoside (MA) is a triterpene derived from Centella asiatica that has been recognized for its antioxidant and anti-inflammatory properties in various disease models. However, its direct impact on cultured white adipocytes and the underlying mechanisms, mainly through gene knockdown, have not been thoroughly explored.<BR><B>METHODS: </B>Western blot analysis was utilized to assess the expression levels of various proteins, while oil red O staining was used to measure lipid deposition. The adipocyte shapes were confirmed using H&E staining.<BR><B>RESULTS: </B>MA treatment enhanced browning and lipolysis in 3T3-L1 adipocytes and adipose tissue from experimental mice while suppressing lipogenesis. Furthermore, MA treatment increased the expression of PPARα and FGF21 in 3T3-L1 adipocytes as well as the secretion of FGF21 into the culture medium. Knockdown of PPARα or FGF21 using siRNA diminished the effects of MA on lipid metabolism in cultured adipocytes.<BR><B>CONCLUSIONS: </B>These findings demonstrate that MA promotes thermogenic browning and lipolysis while inhibiting adipocyte lipogenesis, thus showing the potential for attenuating obesity. The study suggested that MA could be a viable therapeutic approach for treating obesity.