Abstract：
The biocatalytic aerobic \"in-water\" reduction of anthranilic acid to 2-aminobenzaldehyde by growing cultures of the basidiomycetous white-rot fungus Bjerkandera adusta has been studied. The high specific activity of Bjerkandera adusta towards the carboxylic group of anthranilic acid that allows avoiding the formation of the corresponding alcohol has been demonstrated using different substrate concentrations. The presence of ethanol as co-solvent allows increasing the yield of target product. In contrast to chemical reducing agents that usually yield 2-aminobenzyl alcohol, an overreduction of anthranilic acid is completely suppressed by the fungus and gives the target flavor compound in satisfactory preparative yields. It was shown that the activity of Bjerkandera adusta towards anthranilic acid does not apply to its m- and p-isomers.
摘要：
研究了通过培养担子菌白腐真菌Bjerkanderaadusta的培养物，将邻氨基苯甲酸生物催化好氧“水中”还原为2-氨基苯甲醛。已使用不同的底物浓度证明了Bjerkanderaadusta对邻氨基苯甲酸的羧基的高比活性，可以避免形成相应的醇。作为共溶剂的乙醇的存在允许增加目标产物的产率。与通常产生2-氨基苯甲醇的化学还原剂相反，邻氨基苯甲酸的过度还原被真菌完全抑制，并以令人满意的制备产率获得目标风味化合物。研究表明，Bjerkanderaadusta对邻氨基苯甲酸的活性不适用于其m-和p-异构体。
