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Abstract：
The delay in making a correct diagnosis of Candida auris causes concern in the healthcare system setting, and immunoproteomics studies are important to identify immunoreactive proteins for new diagnostic strategies. In this study, immunocompetent murine systemic infections caused by non-aggregative and aggregative phenotypes of C. auris and by Candida albicans and Candida haemulonii were carried out, and the obtained sera were used to study their immunoreactivity against C. auris proteins. The results showed higher virulence, in terms of infection signs, weight loss, and histopathological damage, of the non-aggregative isolate. Moreover, C. auris was less virulent than C. albicans but more than C. haemulonii. Regarding the immunoproteomics study, 13 spots recognized by sera from mice infected with both C. auris phenotypes and analyzed by mass spectrometry corresponded to enolase, phosphoglycerate kinase, glyceraldehyde-3-phosphate dehydrogenase, and phosphoglycerate mutase. These four proteins were also recognized by sera obtained from human patients with disseminated C. auris infection but not by sera obtained from mice infected with C. albicans or Aspergillus fumigatus. Spot identification data are available via ProteomeXchange with the identifier PXD049077. In conclusion, this study showed that the identified proteins could be potential candidates to be studied as new diagnostic or even therapeutic targets for C. auris.
摘要：
对念珠菌进行正确诊断的延迟引起了医疗保健系统设置的关注，和免疫蛋白质组学研究对于鉴定免疫反应蛋白以获得新的诊断策略很重要。在这项研究中,进行了由金黄色葡萄球菌的非聚集和聚集表型以及白色念珠菌和假丝酵母引起的免疫活性小鼠全身感染，并将获得的血清用于研究其对C.auris蛋白的免疫反应性。结果显示毒力较高,就感染迹象而言，减肥,和组织病理学损伤,非聚合隔离的。此外，C.auris的毒力比白色念珠菌小，但比C.haemuronii强。关于免疫蛋白质组学研究，13个斑点由感染两种金丝酵母表型的小鼠的血清识别，并通过质谱分析对应于烯醇化酶，磷酸甘油酸激酶,甘油醛-3-磷酸脱氢酶,和磷酸甘油酸变位酶.这四种蛋白质也被获自播散性金丝酵母感染的人类患者的血清识别,但不被获自感染白色念珠菌或烟曲霉的小鼠的血清识别。点识别数据可通过ProteomeXchange与标识符PXD049077获得。总之,这项研究表明，鉴定出的蛋白质可能是潜在的候选物，可作为C.auris的新诊断甚至治疗靶点进行研究。
