PDF(Pubmed)
Abstract：
Using a combination of short- and long-reads sequencing, we were able to sequence the complete mitochondrial genome of the invasive \'New Zealand flatworm\' Arthurdendyus triangulatus (Geoplanidae, Rhynchodeminae, Caenoplanini) and its two complete paralogous nuclear rRNA gene clusters. The mitogenome has a total length of 20,309 bp and contains repetitions that includes two types of tandem-repeats that could not be solved by short-reads sequencing. We also sequenced for the first time the mitogenomes of four species of Caenoplana (Caenoplanini). A maximum likelihood phylogeny associated A. triangulatus with the other Caenoplanini but Parakontikia ventrolineata and Australopacifica atrata were rejected from the Caenoplanini and associated instead with the Rhynchodemini, with Platydemus manokwari. It was found that the mitogenomes of all species of the subfamily Rhynchodeminae share several unusual structural features, including a very long cox2 gene. This is the first time that the complete paralogous rRNA clusters, which differ in length, sequence and seemingly number of copies, were obtained for a Geoplanidae.
摘要：
使用短读数和长读数测序的组合，我们能够对侵入性\'新西兰扁虫\'Arthurdendyustriangulatus（地球lanidae，Rhynchodeminae,Caenoplanini）及其两个完整的旁系核rRNA基因簇。有丝分裂基因组的总长度为20,309bp，并且包含重复，包括两种类型的串联重复序列，这些重复序列无法通过短读取测序解决。我们还首次对四种Caenoplania（Caenoplanini）的有丝分裂基因组进行了测序。最大可能性的系统发育与其他Caenoplanini相关，但从Caenoplanini中拒绝了Parakontikiaventrolineata和Australopacificaatrata，而与Rhynchodemini相关，和PlatydemusManokwari在一起.发现Rhynchodeminae亚科所有物种的有丝分裂基因组都具有几个不寻常的结构特征，包括一个很长的cox2基因.这是第一次完整的旁系rRNA簇，长度不同，序列和看似数量的副本，是为大地a科获得的。
