PDF(Pubmed)
Abstract：
Fractionating and characterizing target samples are fundamental to the analysis of biomolecules. Extracellular vesicles (EVs), containing information regarding the cellular birthplace, are promising targets for biology and medicine. However, the requirement for multiple-step purification in conventional methods hinders analysis of small samples. Here, we apply a DNA origami tripod with a defined aperture of binders (e.g., antibodies against EV biomarkers), which allows us to capture the target molecule. Using exosomes as a model, we show that our tripod nanodevice can capture a specific size range of EVs with cognate biomarkers from a broad distribution of crude EV mixtures. We further demonstrate that the size of captured EVs can be controlled by changing the aperture of the tripods. This simultaneous selection with the size and biomarker approach should simplify the EV purification process and contribute to the precise analysis of target biomolecules from small samples.
摘要：
分馏和表征目标样品是生物分子分析的基础。细胞外囊泡（EV），包含有关细胞出生地的信息，是生物学和医学的有希望的目标。然而,传统方法中对多步骤纯化的要求阻碍了对小样品的分析。这里,我们应用一个具有确定孔径的粘合剂的DNA折纸三脚架(例如，针对EV生物标志物的抗体），这让我们能够捕获目标分子。使用外泌体作为模型，我们表明,我们的三脚架纳米设备可以从广泛分布的粗EV混合物中捕获特定大小范围的EV与同源生物标志物.我们进一步证明，可以通过改变三脚架的孔径来控制捕获的电动汽车的尺寸。这种具有大小和生物标志物方法的同时选择应简化EV纯化过程并有助于从小样品中精确分析目标生物分子。
