PDF(Pubmed)
Abstract：
Human age estimation from trace samples may give important leads early in a police investigation by contributing to the description of the perpetrator. Several molecular biomarkers are available for the estimation of chronological age, and currently, DNA methylation patterns are the most promising. In this study, a QIAGEN age protocol for age estimation was tested by five forensic genetic laboratories. The assay comprised bisulfite treatment of the extracted DNA, amplification of five CpG loci (in the genes of ELOVL2, C1orf132, TRIM59, KLF14, and FHL2), and sequencing of the amplicons using the PyroMark Q48 platform. Blood samples from 49 individuals with ages ranging from 18 to 64 years as well as negative and methylation controls were analyzed. An existing age estimation model was applied to display a mean absolute deviation of 3.62 years within the reference data set.
UNASSIGNED: Age determination as an intelligence tool during investigations can be a powerful tool in forensic genetics.In this study, five laboratories ran 49 samples and obtained a mean absolute deviation of 3.62 years.Five markers were analyzed on a PyroMark Q48 platform.
UNASSIGNED: Age determination as an intelligence tool during investigations can be a powerful tool in forensic genetics.In this study, five laboratories ran 49 samples and obtained a mean absolute deviation of 3.62 years.Five markers were analyzed on a PyroMark Q48 platform.
摘要：
通过对犯罪者的描述做出贡献，从痕迹样本中估算出的人类年龄可能会在警方调查的早期提供重要线索。几种分子生物标志物可用于估计实际年龄，目前,DNA甲基化模式是最有前途的。在这项研究中,5个法医遗传实验室对用于年龄估计的QIAGEN年龄方案进行了测试.该测定包括亚硫酸氢盐处理提取的DNA，5个CpG基因座的扩增（在ELOVL2，C1orf132，TRIM59，KLF14和FHL2的基因中），和使用PyroMarkQ48平台对扩增子进行测序。分析了49名年龄在18至64岁之间的个体的血液样本以及阴性和甲基化对照。应用现有的年龄估计模型来显示参考数据集中3.62年的平均绝对偏差。
■年龄确定作为调查期间的情报工具可以成为法医遗传学的强大工具。在这项研究中,5个实验室检测了49个样本,得到的平均绝对偏差为3.62年.在PyroMarkQ48平台上分析五个标记。
■年龄确定作为调查期间的情报工具可以成为法医遗传学的强大工具。在这项研究中,5个实验室检测了49个样本,得到的平均绝对偏差为3.62年.在PyroMarkQ48平台上分析五个标记。
