PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to identify a collagen-coating method that does not affect the physicochemical properties of bone graft material. Based on this, we developed a collagen-coated porcine xenograft and applied it to dogs to validate its effectiveness.
UNASSIGNED: Xenografts and collagen were derived from porcine, and the collagen coating was performed through N-ethyl-N\'-(3- (dimethylamino)propyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) activation. The physicochemical characteristics of the developed bone graft material were verified through field emission scanning electron microscope (FE-SEM), brunauer emmett teller (BET), attenuated total reflectance-fourier transform infrared (ATR-FTIR), and water absorption test. Subsequently, the biocompatibility and bone healing effects were assessed using a rat calvarial defect model.
UNASSIGNED: The physicochemical test results confirmed that collagen coating increased bone graft materials\' surface roughness and fluid absorption but did not affect their porous structure. In vivo evaluations revealed that collagen coating had no adverse impact on the bone healing effect of bone graft materials. After confirming the biocompatibility and effectiveness, we applied the bone graft materials in two orthopedic cases and one dental case. Notably, successful fracture healing was observed in both orthopedic cases. In the dental case, successful bone regeneration was achieved without any loss of alveolar bone.
UNASSIGNED: This study demonstrated that porcine bone graft material promotes bone healing in dogs with its hemostatic and cohesive effects resulting from the collagen coating. Bone graft materials with enhanced biocompatibility through collagen coating are expected to be widely used in veterinary clinical practice.
UNASSIGNED: Xenografts and collagen were derived from porcine, and the collagen coating was performed through N-ethyl-N\'-(3- (dimethylamino)propyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) activation. The physicochemical characteristics of the developed bone graft material were verified through field emission scanning electron microscope (FE-SEM), brunauer emmett teller (BET), attenuated total reflectance-fourier transform infrared (ATR-FTIR), and water absorption test. Subsequently, the biocompatibility and bone healing effects were assessed using a rat calvarial defect model.
UNASSIGNED: The physicochemical test results confirmed that collagen coating increased bone graft materials\' surface roughness and fluid absorption but did not affect their porous structure. In vivo evaluations revealed that collagen coating had no adverse impact on the bone healing effect of bone graft materials. After confirming the biocompatibility and effectiveness, we applied the bone graft materials in two orthopedic cases and one dental case. Notably, successful fracture healing was observed in both orthopedic cases. In the dental case, successful bone regeneration was achieved without any loss of alveolar bone.
UNASSIGNED: This study demonstrated that porcine bone graft material promotes bone healing in dogs with its hemostatic and cohesive effects resulting from the collagen coating. Bone graft materials with enhanced biocompatibility through collagen coating are expected to be widely used in veterinary clinical practice.
摘要：
■本研究旨在确定一种不影响骨移植材料的理化性质的胶原蛋白涂层方法。基于此,我们开发了一种胶原蛋白包被的猪异种移植物,并将其应用于狗以验证其有效性.
■异种移植物和胶原蛋白来自猪，并且通过N-乙基-N'-(3-(二甲基氨基)丙基)碳二亚胺/N-羟基琥珀酰亚胺(EDC/NHS)活化进行胶原包被。通过场发射扫描电子显微镜（FE-SEM）验证了开发的植骨材料的理化特性，布鲁诺埃米特出纳员(BET)，衰减全反射-傅里叶变换红外(ATR-FTIR),和吸水试验。随后，使用大鼠颅骨缺损模型评估生物相容性和骨愈合效果.
■物理化学测试结果证实，胶原蛋白涂层增加了骨移植材料\'的表面粗糙度和流体吸收，但不影响其多孔结构。体内评估表明，胶原蛋白涂层对骨移植材料的骨愈合效果没有不利影响。在确认生物相容性和有效性后，我们在两个骨科病例和一个牙科病例中应用了骨移植材料。值得注意的是,在两个骨科病例中均观察到骨折成功愈合。在牙科案例中，成功实现了骨再生，而牙槽骨没有任何损失。
■这项研究表明，猪骨移植材料具有胶原蛋白涂层产生的止血和内聚作用，可促进狗的骨愈合。通过胶原蛋白涂层增强生物相容性的骨移植材料有望广泛用于兽医临床实践。
■异种移植物和胶原蛋白来自猪，并且通过N-乙基-N'-(3-(二甲基氨基)丙基)碳二亚胺/N-羟基琥珀酰亚胺(EDC/NHS)活化进行胶原包被。通过场发射扫描电子显微镜（FE-SEM）验证了开发的植骨材料的理化特性，布鲁诺埃米特出纳员(BET)，衰减全反射-傅里叶变换红外(ATR-FTIR),和吸水试验。随后，使用大鼠颅骨缺损模型评估生物相容性和骨愈合效果.
■物理化学测试结果证实，胶原蛋白涂层增加了骨移植材料\'的表面粗糙度和流体吸收，但不影响其多孔结构。体内评估表明，胶原蛋白涂层对骨移植材料的骨愈合效果没有不利影响。在确认生物相容性和有效性后，我们在两个骨科病例和一个牙科病例中应用了骨移植材料。值得注意的是,在两个骨科病例中均观察到骨折成功愈合。在牙科案例中，成功实现了骨再生，而牙槽骨没有任何损失。
■这项研究表明，猪骨移植材料具有胶原蛋白涂层产生的止血和内聚作用，可促进狗的骨愈合。通过胶原蛋白涂层增强生物相容性的骨移植材料有望广泛用于兽医临床实践。
