PDF(Pubmed)
Abstract：
Raoultella planticola is an emerging bacterial pathogen responsible for causing infections in both humans and animals. Unfortunately, sporadic reports of carbapenem-resistant R. planticola (CRRP) have been documented worldwide. Here we first reported the complete genome sequence of a CRRP isolate RP_3045 co-carrying blaIMP-4 and blaSHV-12, recovered from a patient in China, and its genetic relatedness to 82 R. planticola strains deposited in the NCBI GenBank database, sourced from humans, animals, and the environment. Whole-genome sequencing was performed using the Illumina NovaSeq 6000 and Oxford Nanopore MinION platforms. Phylogenetic analysis was also performed and visualized using a single nucleotide polymorphism (SNP)-based strategy. The complete genome of R. planticola strain RP_3045 was determined to be 6,312,961 bp in length, comprising five contigs that included one chromosome and four plasmids. RP_3045 was found to be multidrug-resistant and harbored several antimicrobial resistance genes, including both blaIMP-4 and blaSHV-12 genes located on a single plasmid. The most closely related strain was hkcpe63, recovered from humans in Hong Kong, China, in 2014, with 506 SNP differences. R. planticola strains were distributed globally and exhibited strong associations among isolates obtained from different sectors. This study provides evidence for the potential of R. planticola to disseminate carbapenem resistance across different sectors, highlighting the critical need for active and continuous surveillance of CRRP.
摘要：
扁平Raoultella是一种新兴的细菌病原体，可引起人类和动物感染。不幸的是,全球范围内都有零星的耐碳青霉烯R.planticola（CRRP）报告。在这里，我们首先报道了从中国一名患者中回收的CRRP分离株RP_3045的完整基因组序列，该分离株共携带blaIMP-4和blaSHV-12，以及它与保存在NCBIGenBank数据库中的82株平头弧菌菌株的遗传相关性，来自人类，动物,和环境。使用IlluminaNovaSeq6000和OxfordNanoporeMinION平台进行全基因组测序。还使用基于单核苷酸多态性(SNP)的策略进行系统发育分析和可视化。经测定，该菌株RP_3045的完整基因组长度为6,312,961bp，包含五个重叠群，其中包括一个染色体和四个质粒。发现RP_3045具有多重耐药性，并具有多个抗菌药物抗性基因，包括位于单个质粒上的blaIMP-4和blaSHV-12基因。最密切相关的菌株是在香港从人类身上回收的hkcpe63，中国,2014年，SNP差异为506。R.planticola菌株分布在全球，并且在从不同部门获得的分离株之间表现出很强的相关性。这项研究提供了证据表明，在不同的部门，R.planticola传播碳青霉烯抗性的潜力，强调了对CRRP进行积极和持续监测的迫切需要。
