PDF(Pubmed)
Abstract：
Disease outbreaks negatively affect fish production. Antimicrobial agents used in the treatment of diseases become ineffective over time because of antibiotic resistance developed by bacteria distributed in the aquaculture environment. This study was conducted for 4 months (cold period) in a fish farm to detect the fish disease, cold water streptococcosis. In the study, four brood stock showing disease signs were detected. Bacteria isolates were obtained and identified as Vagococcus salmoninarum. Antimicrobial susceptibility of V. salmoninarum was tested and antibiotic resistance gene profiles of V. salmoninarum isolates were screened. The phylogenetic relation of the isolates with the previously reported strains was evaluated. Antibiotic resistance developed by pathogenic bacteria is distributed in the aquaculture environment. The transfer of resistance genes from one bacterium to another is very common. This situation causes the antimicrobial agents used in the treatment of diseases to become ineffective over time. The disc diffusion test showed that all four isolates developed resistance to 13 (FFC30, AX25, C30, E15, CF30, L2, OX1, S10, T30, CRO30, CC2, PT15 and TY15) of the evaluated antibiotics and were about to develop resistance to six others (AM 10, FM 300, CFP75, SXT25, APR15 and TE30). Furthermore, antibiotic resistance genes tetA, sul1, sul2, sul3, dhfr1, ereB and floR were detected in the isolated strain. Moreover, the phylogenetic analysis showed that isolated V. salmoninarum strain (ESN1) was closely related to the bacterial strains isolated from USA and Jura.
摘要：
疾病爆发对鱼类生产产生负面影响。由于分布在水产养殖环境中的细菌产生的抗生素抗性,用于治疗疾病的抗微生物剂随着时间的推移变得无效。这项研究在一个养鱼场进行了4个月（寒冷时期），以检测鱼病，冷水链球菌病.在研究中,检测到四只显示疾病迹象的育苗。获得细菌分离物，并鉴定为沙门氏菌阴道球菌。检测了沙门氏菌的药敏性，并筛选了沙门氏菌分离株的抗生素抗性基因谱。评估了分离株与先前报道的菌株的系统发育关系。病原菌产生的抗生素耐药性分布在水产养殖环境中。抗性基因从一种细菌转移到另一种细菌是非常常见的。这种情况导致用于治疗疾病的抗微生物剂随着时间的推移变得无效。圆盘扩散测试表明，所有四个分离株都对所评估抗生素的13种（FFC30，AX25，C30，E15，CF30，L2，OX1，S10，T30，CRO30，CC2，PT15和TY15）产生了耐药性。对其他六种（AM10，FM300，CFP75，SXT25，APR15和TE30）产生了耐药性。此外，抗生素抗性基因tetA，在分离的菌株中检测到sul1，sul2，sul3，dhfr1，ereB和floR。此外，系统发育分析表明，分离的沙门氏菌菌株（ESN1）与美国和Jura分离的细菌菌株密切相关。
