PDF(Pubmed)
Abstract：
Balantidium ctenopharyngodoni is identified as the sole ciliate species that exclusively resides within the hindgut of grass carp with high prevalence and intensity. In this study, the successful cultivation of B. ctenopharyngodoni enabled us to collect enough cells for genome sequencing. Consequently, we acquired a high-quality genome assembly spanning 68.66 Mb, encompassing a total of 22,334 nanochromosomes. Furthermore, we predicted 29,348 protein-coding genes, and 95.5% of them was supported by the RNA-seq data. The trend of GC content in the subtelomeric regions of single-gene chromosomes was similar to other ciliates containing nanochromosomes. A large number of genes encoding carbohydrate-binding modules with affinities for starch and peptidoglycans was identified. The identification of mitochondrion-related organelles (MROs) within genome indicates its well-suited adaptation to the anaerobic conditions in the hindgut environment. In summary, our results will offer resources for understanding the genetic basis and molecular adaptations of balantidia to hindgut of herbivorous fish.
摘要：
ctenopharngodoniBalantidium被确定为唯一的纤毛虫物种，仅位于草鱼的后肠内，具有很高的患病率和强度。在这项研究中,B.ctenopharyngodoni的成功培养使我们能够收集足够的细胞进行基因组测序。因此,我们获得了68.66Mb的高质量基因组组装，总共包含22,334个纳米染色体。此外，我们预测了29,348个蛋白质编码基因，其中95.5%得到了RNA-seq数据的支持。单基因染色体亚端粒区GC含量的变化趋势与其他含纳米染色体的纤毛虫相似。鉴定了大量编码对淀粉和肽聚糖具有亲和力的碳水化合物结合模块的基因。基因组中线粒体相关细胞器（MRO）的鉴定表明其非常适合后肠环境中的厌氧条件。总之,我们的研究结果将提供资源，以了解balantidia的遗传基础和分子适应食草鱼的后肠。
