Abstract：
α-DCs (α-dicarbonyls) have been proven to be closely related to aging and the onset and development of many chronic diseases. The wide presence of this kind of components in various foods and beverages has been unambiguously determined, but their occurrence in various phytomedicines remains in obscurity. In this study, we established and evaluated an HPLC-UV method and used it to measure the contents of four α-DCs including 3-deoxyglucosone (3-DG), glyoxal (GO), methylglyoxal (MGO), and diacetyl (DA) in 35 Chinese herbs after they have been derivatized with 4-nitro-1,2-phenylenediamine. The results uncover that 3-DG is the major component among the α-DCs, being detectable in all the selected herbs in concentrations ranging from 22.80 μg/g in the seeds of Alpinia katsumadai to 7032.75 μg/g in the fruit of Siraitia grosuenorii. The contents of the other three compounds are much lower than those of 3-DG, with GO being up to 22.65 μg/g, MGO being up to 55.50 μg/g, and DA to 18.75 μg/g, respectively. The data show as well the contents of the total four α-DCs in the herbs are generally in a comparable level to those in various foods, implying that herb medicines may have potential risks on human heath in view of the α-DCs.
摘要：
α-DCs（α-二羰基）已被证明与衰老和许多慢性疾病的发生和发展密切相关。这类成分在各种食品和饮料中的广泛存在已被明确确定，但是它们在各种植物药中的出现仍然默默无闻。在这项研究中,我们建立并评估了HPLC-UV方法，并将其用于测量包括3-脱氧葡萄糖酮（3-DG）在内的四种α-DC的含量，乙二醛（GO），甲基乙二醛（MGO），和35种中草药中的二乙酰（DA）用4-硝基-1,2-苯二胺衍生化。结果发现3-DG是α-DC中的主要成分，在所有选定的草药中都可检测到，浓度范围从高良姜种子中的22.80μg/g到西兰果实中的7032.75μg/g。其它三种化合物的含量远低于3-DG,GO高达22.65μg/g，MGO高达55.50μg/g，和DA至18.75μg/g，分别。数据显示，草药中总共四种α-DC的含量通常与各种食物中的含量相当，这意味着，鉴于α-DCs，草药可能对人类健康有潜在风险。
