Abstract：
Staphylococcus pseudintermedius is the most common cause of pyoderma in dogs. We validated a point-of-care (PoC) test based on colorimetric loop-mediated isothermal amplification (LAMP) for rapid S. pseudintermedius identification and susceptibility testing for first line antimicrobials for systemic treatment of canine pyoderma, i.e., lincosamides, first generation cephalosporins and amoxicillin clavulanate. Newly designed LAMP primers targeting clinically relevant resistance genes were combined with a previously validated set of primers targeting spsL for species identification. After laboratory validation on 110 clinical isolates, we assessed the performance of the test on 101 clinical specimens using routine culture and susceptibility testing as a reference standard. The average hands-on and turnaround times for the PoC test were 30 and 90 min, respectively. The assay showed sensitivity and specificity near 100% for both species identification and susceptibility testing when performed on bacterial cultures or clinical specimens in the laboratory. However, the PoC test yielded less accurate results when performed on-site by clinical staff (92% sensitivity and 64% specificity for species identification, 67% sensitivity and 96% specificity for β-lactam susceptibility, and 83% sensitivity and 71% specificity for lincosamide susceptibility). These results indicate that the PoC test should be adapted to a user-friendly technology to facilitate performance and interpretation of results by clinical staff. If properly developed, the test would allow veterinarians to gain rapid information on antimicrobial choice, limiting the risk of treatment failure and facilitating adherence to antimicrobial use guidelines in small animal veterinary dermatology.
摘要：
假中间葡萄球菌是狗脓皮病的最常见原因。我们验证了基于比色环介导等温扩增（LAMP）的即时护理（PoC）测试，用于快速鉴定假中介杆菌，并对一线抗生素进行敏感性测试，以全身治疗犬脓皮病，即，lincosamides,第一代头孢菌素和克拉维酸阿莫西林。将新设计的靶向临床相关抗性基因的LAMP引物与先前验证的靶向spsL的引物组合用于物种鉴定。经过110个临床分离株的实验室验证，我们使用常规培养和药敏试验作为参考标准,对101份临床标本进行了检测.PoC测试的平均操作和周转时间为30和90分钟，分别。当在实验室的细菌培养物或临床标本上进行时，该测定法显示出物种鉴定和敏感性测试的敏感性和特异性接近100％。然而,当临床工作人员在现场进行时，PoC测试产生的结果不太准确(物种鉴定的灵敏度为92%，特异性为64%，对β-内酰胺的敏感性为67%，特异性为96%，lincosamide敏感性为83%，特异性为71%）。这些结果表明，PoC测试应适应用户友好的技术，以促进临床人员对结果的执行和解释。如果开发得当,该测试将使兽医能够快速获得抗菌药物选择的信息，限制治疗失败的风险，并促进小动物兽医皮肤病学抗菌药物使用指南的遵守。
