PDF(Pubmed)
Abstract：
Surface waters are considered ecological habitats where Salmonella enterica can persist and disseminate to fresh produce production systems. This study aimed to explore the genomic profiles of S. enterica serotypes Typhimurium, Newport, and Infantis from surface waters in Chile, Mexico, and Brazil collected between 2019 and 2022. We analyzed the whole genomes of 106 S. Typhimurium, 161 S. Newport, and 113 S. Infantis isolates. Our phylogenetic analysis exhibited distinct groupings of isolates by their respective countries except for a notable case involving a Chilean S. Newport isolate closely related to two Mexican isolates, showing 4 and 13 single nucleotide polymorphisms of difference, respectively. The patterns of the most frequently detected antimicrobial resistance genes varied across countries and serotypes. A strong correlation existed between integron carriage and genotypic multidrug resistance (MDR) across serotypes in Chile and Mexico (R > 0.90, P < 0.01), while integron(s) were not detected in any of the Brazilian isolates. By contrast, we did not identify any strong correlation between plasmid carriage and genotypic MDR across diverse countries and serotypes.IMPORTANCEUnveiling the genomic landscape of S. enterica in Latin American surface waters is pivotal for ensuring public health. This investigation sheds light on the intricate genomic diversity of S. enterica in surface waters across Chile, Mexico, and Brazil. Our research also addresses critical knowledge gaps, pioneering a comprehensive understanding of surface waters as a reservoir for multidrug-resistant S. enterica. By integrating our understanding of integron carriage as biomarkers into broader MDR control strategies, we can also work toward targeted interventions that mitigate the emergence and dissemination of MDR in S. enterica in surface waters. Given its potential implications for food safety, this study emphasizes the critical need for informed policies and collaborative initiatives to address the risks associated with S. enterica in surface waters.
摘要：
地表水被认为是肠道沙门氏菌可以持续存在并传播到新鲜农产品生产系统的生态栖息地。本研究旨在探索鼠伤寒沙门氏菌血清型的基因组图谱，纽波特,来自智利地表水的婴儿，墨西哥,和巴西在2019年至2022年之间收集。我们分析了106种鼠伤寒沙门氏菌的全基因组,161S.纽波特，和113个婴儿分离株。我们的系统发育分析显示，除了涉及与两个墨西哥分离株密切相关的智利S.Newport分离株的著名病例外，它们各自国家的分离株分组不同。显示4和13个单核苷酸多态性的差异，分别。最常检测到的抗菌素耐药基因的模式因国家和血清型而异。在智利和墨西哥的血清型中，整合子携带与基因型多药耐药（MDR）之间存在很强的相关性（R>0.90，P<0.01），而在任何巴西分离株中均未检测到整合子。相比之下,我们没有发现不同国家和血清型的质粒携带和基因型MDR之间有任何强相关性.重要的是，在拉丁美洲地表水中揭开S.enterica的基因组景观对于确保公共卫生至关重要。这项调查揭示了智利地表水中肠道链球菌复杂的基因组多样性，墨西哥,和巴西。我们的研究还解决了关键的知识差距，开创了对地表水作为多重耐药肠球菌的水库的全面了解。通过将我们对整合子携带作为生物标志物的理解整合到更广泛的MDR控制策略中，我们还可以采取有针对性的干预措施，以减轻地表水中肠道链球菌中MDR的出现和传播。鉴于其对食品安全的潜在影响，这项研究强调了对知情政策和合作举措的迫切需要,以解决地表水中与肠球菌相关的风险.
