Abstract：
Efficient extraction of natural pigments is a key focus in enhancing the utilization of by-products for applications in the food industry. In this study, an enzymatic extraction method using Pectinex Ultra SP-L, Pectinex XXL, Novoshape, and Celluclast was used to investigate natural pigment production from the pomace of aronia, a commercially important plant. The method\'s performance was monitored using high-performance liquid chromatography with diode-array detection by measuring total and individual anthocyanin levels. Pectinex XXL (0.5%) yielded the highest total anthocyanin extraction (2082.41 ± 85.69 mg/100 g) in the single enzyme treatment, followed by Pectinex Ultra SP-L (0.05%), Celluclast (0.01%), and Novoshape (0.1%). Combining Pectinex XXL (0.25%) with Celluclast (0.01%) increased the extraction ratio of total anthocyanins (2 323.04 ± 61.32 mg/100 g) by ∼50.7% compared with that obtained using the solvent extraction method. This study demonstrated an effective enzymatic extraction method for application in the food industry.
摘要：
天然色素的有效提取是提高食品工业中应用的副产品利用率的关键焦点。在这项研究中,使用PectinexUltraSP-L的酶法提取，PectinexXXL,Novoshape,Celluclast被用来研究从阿拉伯果渣中产生的天然色素，一个商业上重要的工厂。使用具有二极管阵列检测的高效液相色谱法通过测量总花青素和个体花青素水平来监测方法的性能。PectinexXXL（0.5％）在单一酶处理中产生最高的总花色苷提取量（2082.41±85.69mg/100g），其次是PectinexUltraSP-L（0.05％），Celluclast(0.01%),和新形状（0.1%）。与使用溶剂萃取法相比，将PectinexXXL（0.25％）与Celluclast（0.01％）结合使用使总花色苷的提取率（2323.04±61.32mg/100g）增加了〜50.7％。这项研究证明了一种在食品工业中应用的有效的酶法提取方法。
