PDF(Pubmed)
Abstract：
Natural product (NP)-based pesticides have emerged as a compelling alternative to traditional chemical fungicides, attracting substantial attention within the agrochemical industry as the world is pushing toward sustainable and environmentally friendly approaches to safeguard crops. Microbes, both bacteria and fungi, are a huge source of diverse secondary metabolites with versatile applications across pharmaceuticals, agriculture, and the food industry. Microbial genome mining has been accelerated for pesticide/drug discovery and development in recent years, driven by advancements in genome sequencing, bioinformatics, metabolomics/metabologenomics, and synthetic biology. Here, we isolated and identified Pseudomonas vancouverensis that had shown antifungal activities against crop fungal pathogens Colletotrichum fragariae, Botrytis cinerea, and Phomopsis obscurans in a dual-plate culture and bioautography assay. Further, we sequenced the whole bacterial genome and mined the genome of this bacterium to identify secondary metabolite biosynthetic gene clusters (BGCs) using antiSMASH 7.0, PRISM 4, and BAGEL 4. An in-silico analysis suggests that P. vancouverensis possesses a rich repertoire of BGCs with the potential to produce diverse and novel NPs, including non-ribosomal peptides (NRPs), polyketides (PKs), acyl homoserine lactone, cyclodipeptide, bacteriocins, and ribosomally synthesized and post-transcriptionally modified peptides (RiPPs). Bovienimide-A, an NRP, and putidacin L1, a lectin-like bacteriocin, were among the previously known predicted metabolites produced by this bacterium, suggesting that the NPs produced by this bacterium could have biological activities and be novel as well. Future studies on the antifungal activity of these compounds will elucidate the full biotechnological potential of P. vancouverensis.
摘要：
基于天然产物（NP）的农药已成为传统化学杀菌剂的引人注目的替代品，由于世界正在推动可持续和环境友好的方法来保护作物，因此在农用化学工业中引起了极大的关注。微生物,细菌和真菌，是多种次生代谢物的巨大来源，在药物中具有广泛的应用，农业,和食品工业。近年来，微生物基因组挖掘已加速用于农药/药物的发现和开发，在基因组测序进步的推动下，生物信息学，代谢组学/代谢基因组学，和合成生物学。这里,我们分离并鉴定了对作物真菌病原体具有抗真菌活性的假单胞菌，灰葡萄孢菌,在双板培养和生物自图分析中，Phomopsis变得模糊。Further,我们使用抗SMASH7.0，PRISM4和BAGEL4对整个细菌基因组进行了测序，并挖掘了该细菌的基因组，以鉴定次级代谢产物生物合成基因簇（BGC）。计算机分析显示，vancouverensis拥有丰富的BGC库，有可能产生多样化和新颖的NP，包括非核糖体肽（NRP），聚酮化合物(PKs)，酰基高丝氨酸内酯，环二肽，细菌素,和核糖体合成和转录后修饰的肽(RiPPs)。Bovienimide-A,NRP,和普蒂丁L1，一种凝集素样细菌素，是这种细菌产生的先前已知的预测代谢物之一，表明该细菌产生的NPs可能具有生物学活性并且也是新颖的。对这些化合物的抗真菌活性的未来研究将阐明P.vancouverensis的全部生物技术潜力。
