PDF(Pubmed)
Abstract：
Climate change, particularly drought stress, significantly impacts plant growth and development, necessitating the development of resilient crops. This study investigated physiological and molecular modulations to drought stress between diploid parent species and their polyploid progeny in the Brassica species. While no significant phenotypic differences were observed among the six species, drought stress reduced growth parameters by 2.4% and increased oxidative stress markers by 1.4-fold. Drought also triggered the expression of genes related to stress responses and led to the accumulation of specific metabolites. We also conducted the first study of perfluorooctane sulfonic acid (PFOS) levels in leaves as a drought indicator. Lower levels of PFOS accumulation were linked to plants taking in less water under drought conditions. Both diploid and polyploid species responded to drought stress similarly, but there was a wide range of variation in their responses. In particular, responses were less variable in polyploid species than in diploid species. This suggests that their additional genomic components acquired through polyploidy may improve their flexibility to modulate stress responses. Despite the hybrid vigor common in polyploid species, Brassica polyploids demonstrated intermediate responses to drought stress. Overall, this study lays the framework for future omics-level research, including transcriptome and proteomic studies, to deepen our understanding of drought tolerance mechanisms in Brassica species.
摘要：
气候变化,特别是干旱胁迫，显着影响植物的生长和发育，需要发展有弹性的作物。这项研究调查了芸苔属物种中二倍体亲本物种及其多倍体后代之间对干旱胁迫的生理和分子调节。虽然在六个物种之间没有观察到显着的表型差异，干旱胁迫使生长参数降低了2.4％，氧化应激标记物增加了1.4倍。干旱还触发了与应激反应相关的基因的表达，并导致特定代谢产物的积累。我们还对叶片中的全氟辛烷磺酸（PFOS）水平作为干旱指标进行了首次研究。全氟辛烷磺酸积累水平较低与植物在干旱条件下吸收较少的水有关。二倍体和多倍体物种对干旱胁迫的反应相似，但是他们的反应差异很大。特别是,与二倍体物种相比，多倍体物种的响应变化较小。这表明，通过多倍体获得的其他基因组成分可能会提高其调节应激反应的灵活性。尽管多倍体物种普遍存在杂种活力，芸苔属多倍体表现出对干旱胁迫的中等响应。总的来说,这项研究为未来的组学研究奠定了框架，包括转录组和蛋白质组研究，以加深我们对芸苔属植物耐旱机制的理解。
