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Abstract：
Fiber diameter is an important characteristic that determines the quality and economic value of rabbit wool. This study aimed to investigate the genetic determinants of wool fiber diameter through an integration analysis using transcriptomic and proteomic datasets from hair follicles of coarse and fine wool from Angora rabbits. Using a 4D label-free technique, we identified 423 differentially expressed proteins (DEPs) in hair follicles of coarse and fine wool in Angora rabbits. Eighteen DEPs were examined using parallel reaction monitoring, which verified the reliability of our proteomic data. Functional enrichment analysis revealed that a set of biological processes and signaling pathways related to wool growth and hair diameter were strongly enriched by DEPs with fold changes greater than two, such as keratinocyte differentiation, skin development, epidermal and epithelial cell differentiation, epidermis and epithelium development, keratinization, and estrogen signaling pathway. Association analysis and protein-protein interaction network analysis further showed that the keratin (KRT) family members, including KRT77, KRT82, KRT72, KRT32, and KRT10, as well as CASP14 and CDSN, might be key factors contributing to differences in fiber diameter. Our results identified DEPs in hair follicles of coarse and fine wool and promoted understanding of the molecular mechanisms underlying wool fiber diameter variation among Angora rabbits.
摘要：
纤维直径是决定兔羊毛质量和经济价值的重要特征。本研究旨在通过使用来自安哥拉兔粗羊毛和细羊毛毛囊的转录组和蛋白质组数据集的整合分析来研究羊毛纤维直径的遗传决定因素。使用4D无标签技术，我们在安哥拉兔粗毛和细毛毛囊中鉴定出423种差异表达蛋白（DEP）。使用平行反应监测检查了18个DEP，这验证了我们的蛋白质组数据的可靠性。功能富集分析显示，一组与羊毛生长和头发直径相关的生物过程和信号通路被DEP强烈富集，倍数变化大于2，如角质形成细胞分化，皮肤发育,表皮和上皮细胞分化，表皮和上皮发育，角质化,和雌激素信号通路。关联分析和蛋白质-蛋白质相互作用网络分析进一步表明,角蛋白(KRT)家族成员,包括KRT77、KRT82、KRT72、KRT32和KRT10，以及CASP14和CDSN，可能是导致纤维直径差异的关键因素。我们的结果确定了粗羊毛和细羊毛毛囊中的DEP，并促进了对安哥拉兔羊毛纤维直径变化的分子机制的理解。
