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Abstract：
Laccase from Trametes versicolor was applied to produce phenolic polymeric compounds with enhanced properties, using a wine lees extract as the phenolic source. The influence of the incubation time on the progress of the enzymatic oxidation and the yield of the formed polymers was examined. The polymerization process and the properties of the polymeric products were evaluated with a variety of techniques, such as high-pressure liquid chromatography (HPLC) and gel permeation chromatography (GPC), Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopies, differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). The enzymatic polymerization reaction resulted in an 82% reduction in the free phenolic compounds of the extract. The polymeric product recovery (up to 25.7%) and the molecular weight of the polymer depended on the incubation time of the reaction. The produced phenolic polymers exhibited high antioxidant activity, depending on the enzymatic oxidation reaction time, with the phenolic polymer formed after one hour of enzymatic reaction exhibiting the highest antioxidant activity (133.75 and 164.77 μg TE mg-1 polymer) towards the ABTS and DPPH free radicals, respectively. The higher thermal stability of the polymeric products compared to the wine lees phenolic extract was confirmed with TGA and DSC analyses. Finally, the formed phenolic polymeric products were incorporated into chitosan films, providing them with increased antioxidant activity without affecting the films\' cohesion.
摘要：
来自Trametesversicolor的漆酶用于生产具有增强性能的酚类聚合物，使用酒糟提取物作为酚类来源。考核了孵育时光对酶促氧化进程和所形成聚合物产率的影响。用多种技术对聚合过程和聚合产物的性能进行了评价,如高压液相色谱(HPLC)和凝胶渗透色谱(GPC)，傅里叶变换红外（FTIR）和核磁共振（NMR）光谱，差示扫描量热法(DSC),和热重分析(TGA)。酶促聚合反应导致提取物的游离酚类化合物减少82%。聚合物产物的回收率（高达25.7％）和聚合物的分子量取决于反应的孵育时间。所生产的酚类聚合物表现出较高的抗氧化活性,取决于酶促氧化反应时间，酶促反应一小时后形成的酚类聚合物对ABTS和DPPH自由基表现出最高的抗氧化活性（133.75和164.77μgTEmg-1聚合物），分别。用TGA和DSC分析证实了与酒糟酚类提取物相比,聚合物产品具有更高的热稳定性。最后,将形成的酚类聚合物产物掺入壳聚糖膜中，为它们提供增加的抗氧化活性而不影响薄膜的内聚力。
