PDF(Pubmed)
Abstract：
X-ray irradiation and modified atmospheres (MAs) provide eco-friendly, chemical-free methods for pest management. Although a low-oxygen atmospheric treatment improves the performance of some irradiated insects, its influence on the irradiation of quarantine insects and its impacts on pest control efficacy have yet to be investigated. Based on bioassay results, this study employed direct immersion solid-phase microextraction (DI-SPME) combined with gas chromatography-mass spectrometry (GC-MS) to determine metabolic profiles of late third-instar B. dorsalis larvae under normoxia (CON, Air), hypoxia (95% N2 + 5% O2, HY), super-hypoxia (99.5% N2 + 0.5% O2, Sup-HY), irradiation-alone (116 Gy, IR-alone), hypoxia + irradiation (HY + IR) and super-hypoxia + irradiation (Sup-HY + IR). Our findings reveal that, compared to the IR-alone group, the IR treatment under HY and Sup-HY (HY + IR and Sup-HY + IR) increases the larval pupation of B. dorsalis, and weakens the delaying effect of IR on the larval developmental stage. However, these 3 groups further hinder adult emergence under the phytosanitary IR dose of 116 Gy. Moreover, all IR-treated groups, including IR-alone, HY + IR, and Sup-HY + IR, lead to insect death as a coarctate larvae or pupae. Pathway analysis identified changed metabolic pathways across treatment groups. Specifically, changes in lipid metabolism-related pathways were observed: 3 in HY vs. CON, 2 in Sup-HY vs. CON, and 5 each in IR-alone vs. CON, HY + IR vs. CON, and Sup-HY + IR vs. CON. The treatments of IR-alone, HY + IR, and Sup-HY + IR induce comparable modifications in metabolic pathways. However, in the HY + IR, and Sup-HY + IR groups, the third-instar larvae of B. dorsalis demonstrate significantly fewer changes. Our research suggests that a low-oxygen environment (HY and Sup-HY) might enhance the radiation tolerance in B. dorsalis larvae by stabilizing lipid metabolism pathways at biologically feasible levels. Additionally, our findings indicate that the current phytosanitary IR dose contributes to the effective management of B. dorsalis, without being influenced by radioprotective effects. These results hold significant importance for understanding the biological effects of radiation on B. dorsalis and for developing IR-specific regulatory guidelines under MA environments.
摘要：
X射线辐照和改性气氛（MA）提供环保，无化学品害虫管理方法。尽管低氧大气处理改善了一些受辐照昆虫的性能，其对检疫昆虫辐照的影响及其对害虫防治效果的影响尚待研究。根据生物测定结果,本研究采用直接浸没固相微萃取（DI-SPME）结合气相色谱-质谱（GC-MS）来确定常氧下晚三龄B.dorsalis幼虫的代谢谱（CON，空气),缺氧(95%N2+5%O2,HY),超缺氧(99.5%N2+0.5%O2,Sup-HY),单独照射(116Gy，IR-单独),缺氧+照射(HY+IR)和超缺氧+照射(Sup-HY+IR)。我们的发现表明,与单独的IR组相比，HY和Sup-HY（HYIR和Sup-HYIR）下的IR处理增加了背芽孢杆菌的幼虫化p，并减弱了IR对幼虫发育阶段的延缓作用。然而,这3组进一步妨碍了植物检疫IR剂量为116Gy下的成虫出苗。此外，所有IR处理组，包括单独的IR，HY+IR,和Sup-HY+IR，导致昆虫死亡，如结骨幼虫或蛹。途径分析确定了治疗组之间代谢途径的变化。具体来说,观察到脂质代谢相关途径的变化：3在HY与CON,2inSup-HYvs.CON,和5个单独的IR与CON,HY+IRvs.CON,和Sup-HY+IRvs.CON.单独IR的治疗，HY+IR,和Sup-HY+IR在代谢途径中诱导相当的修饰。然而,在HY+IR中，和Sup-HY+IR基团，背芽孢杆菌的三龄幼虫表现出明显较少的变化。我们的研究表明，低氧环境（HY和Sup-HY）可能通过将脂质代谢途径稳定在生物学上可行的水平来增强背芽孢杆菌幼虫的辐射耐受性。此外,我们的发现表明，目前的植物检疫IR剂量有助于有效管理背芽孢杆菌，不受辐射防护作用的影响。这些结果对于理解辐射对背芽孢杆菌的生物学效应以及在MA环境下制定IR特异性调控指南具有重要意义。
