PDF(Pubmed)
Abstract：
The house fly, Musca domestica L., is a significant human and livestock pest. Experiments used female adult house flies glued onto toothpicks for controlled exposure of their tarsi alone (tarsal assay) or their tarsi and proboscis (proboscis assay) with a sucrose solution containing imidacloprid at either a low (10 µg/mL) or high (4000 µg/mL) concentration. Proboscis extension response (PER) assays were used to characterize the response of imidacloprid-susceptible and behaviorally resistant house fly strains to contact with sucrose solutions containing either a low or high concentration of imidacloprid. In each assay, 150 female flies from each fly strain were individually exposed to sucrose solutions containing either a low or high concentration of imidacloprid by deliberate contact of the fly tarsi to the test solution. The PER for each fly was subsequently recorded at 0, 2, and 10 s following the initial tarsal contact. A significant and rapid reduction in PER was observed only for the behaviorally resistant fly strain and only following contact by the flies\' proboscis with the sucrose solution containing the high imidacloprid concentration. The results suggest that chemoreceptors on the fly labellum or internally on the pharyngeal taste organs are involved in the detection of imidacloprid and discrimination of the concentration, resulting in an avoidance behavior (proboscis retraction) only when imidacloprid is at sufficient concentration. Further research is needed to identify the specific receptor(s) responsible for imidacloprid detection.
摘要：
房子飞，家蝇L.，是一种重要的人类和牲畜害虫。实验使用雌性成年苍蝇粘在牙签上，以控制其单独的tarsi（tarsal测定）或tarsi和proboscis（proboscis测定）与含有低（10µg/mL）或高（4000µg/mL）浓度的吡虫啉的蔗糖溶液。Probosc延伸反应（PER）测定用于表征吡虫啉敏感和行为抗性家蝇菌株与含有低浓度或高浓度吡虫啉的蔗糖溶液接触的反应。在每次检测中,通过使蝇tarsi与测试溶液故意接触，将来自每个蝇菌株的150只雌蝇分别暴露于含有低浓度或高浓度吡虫啉的蔗糖溶液。随后在最初的tar骨接触后的0、2和10s记录每个苍蝇的PER。仅在行为抗性蝇菌株中观察到PER显着快速降低，并且仅在蝇\''proboscis与含有高浓度吡虫啉的蔗糖溶液接触后才观察到。结果表明，飞唇上或咽味器官内部的化学感受器参与了吡虫啉的检测和浓度的区分，只有当吡虫啉浓度足够时，才会产生回避行为（长鼻回缩）。需要进一步的研究来鉴定负责吡虫啉检测的特异性受体。
