PDF(Pubmed)
Abstract：
The Colorado potato beetle (Leptinotarsa decemlineata (Say)) can cause extensive damage to agricultural crops worldwide and is a significant insect pest. This insect is notorious for its ability to evade various strategies deployed to control its spread and is known for its relative ease in developing resistance against different insecticides. Various molecular levers are leveraged by L. decemlineata for this resistance to occur, and a complete picture of the genes involved in this process is lacking. While small non-coding RNAs, including miRNAs, are differentially expressed in insects exposed to insecticides, levels of transcript coding for proteins underlying their synthesis remain to be characterized fully. The overarching objective of this work aims to fill that gap by assessing the expression of such targets in L. decemlineata exposed to cyantraniliprole and thiamethoxam. The expression status of Ago1, Ago2, Ago3, Dcr2a, Dcr2b, Expo-5, Siwi-1 and Siwi-2 transcripts were quantified via qRT-PCR in adult L. decemlineata treated with low and high doses of these compounds for different lengths of time. Variation in Ago1 and Dcr2b expression was notably observed in L. decemlineata exposed to cyantraniliprole, while thiamethoxam exposure was associated with the modulation of Dcr2a and Siwi-1 transcript levels. The down-regulation of Ago1 expression in L. decemlineata using dsRNA, followed by cyantraniliprole treatment, was associated with a reduction in the survival of insects with reduced Ago1 transcript expression. Overall, this work presents the insecticide-mediated modulation of transcripts associated with small non-coding RNA processing and showcases Ago1 as a target to further investigate its relevance in cyantraniliprole response.
摘要：
科罗拉多马铃薯甲虫（Leptinotarsadecemlineata（Say））会对全世界的农作物造成广泛的损害，并且是一种重要的害虫。这种昆虫以其逃避控制其传播的各种策略的能力而臭名昭著，并且以其相对容易发展对不同杀虫剂的抗性而闻名。decemlineata利用各种分子杠杆来实现这种抗性，缺乏参与这一过程的基因的完整图片。虽然小的非编码RNA，包括miRNAs,在暴露于杀虫剂的昆虫中差异表达，编码其合成基础蛋白质的转录物水平仍有待充分表征。这项工作的总体目标旨在通过评估暴露于蓝藻脲和噻虫嗪的L.decemlineata中此类靶标的表达来填补这一空白。Ago1、Ago2、Ago3、Dcr2a的表达状态,Dcr2b,通过qRT-PCR在用低剂量和高剂量的这些化合物处理不同时间长度的成年L.decemlineata中定量Expo-5，Siwi-1和Siwi-2转录本。在暴露于cyantraniliprole的L.decemlineata中，显着观察到Ago1和Dcr2b表达的变化，而噻虫嗪暴露与Dcr2a和Siwi-1转录水平的调节有关。使用dsRNA下调Decemlineata中的Ago1表达，其次是氰化氢治疗，与Ago1转录物表达降低的昆虫的存活率降低有关。总的来说,这项工作介绍了杀虫剂介导的与小的非编码RNA加工相关的转录本的调节，并展示了Ago1作为靶标，以进一步研究其在氰化虫反应中的相关性。
