PDF(Pubmed)
Abstract：
The mushroom industry should implement green extraction technologies; however, there is not enough information on the differences between these techniques expressed as the chemical composition of the resulting extract. In this study, selected types of green extraction techniques (GETs) were used on Chaga (Inonotus obliquus) (Fr.) Pilát from Serbia (IS) and Mongolia (IM) to examine the differences that would enable the composition-based technology choices in the mushroom supplement industry. Subcritical water extraction (SWE), microwave-assisted (MW) extraction, and ultrasonic-assisted extraction (VAE) were used to prepare the extracts. SWE was performed at two different temperatures (120 and 200 °C), while 96% ethanol, 50% ethanol, and water were used for MW and VAE. The yield, the content of total phenols, total proteins, and carbohydrates, qualitative and quantitative analysis of phenolic compounds, carbohydrates, including α- and β- and total glucans, and fatty acids, were determined in the obtained extracts. SWE resulted in a significantly higher yield, total polysaccharide, and glucan content than any other technique. Glucose was the most dominant monosaccharide in the SWE samples, especially those extracted at 200 °C. The MW 50% EtOH extracts showed the highest yield of total phenols. Among the tested phenolic compounds, chlorogenic acid was the most dominant. SWE can be recommended as the most efficient method for extracting commercially important compounds, especially glucans and phenols.
摘要：
蘑菇行业应实施绿色提取技术；然而，关于这些技术之间的差异，没有足够的信息表示为所得提取物的化学成分。在这项研究中,在Chaga（Inonotusobrayquus）（Fr。）来自塞尔维亚（IS）和蒙古（IM）的Pilát研究了在蘑菇补充剂行业中实现基于成分的技术选择的差异。亚临界水萃取（SWE），微波辅助（MW）提取，采用超声辅助提取法(VAE)制备提取物。SWE在两个不同的温度（120和200°C）下进行，而96%的乙醇，50%乙醇,和水用于MW和VAE。产量，总酚的含量，总蛋白质，和碳水化合物，酚类化合物的定性和定量分析，碳水化合物,包括α-和β-和总葡聚糖，和脂肪酸，在获得的提取物中测定。SWE导致显著更高的产量，总多糖，和葡聚糖含量比任何其他技术。葡萄糖是SWE样品中最主要的单糖，特别是那些在200°C提取的MW50%EtOH提取物显示出最高的总酚收率。在测试的酚类化合物中，绿原酸是最主要的。SWE可被推荐为提取商业上重要化合物的最有效方法，尤其是葡聚糖和酚类.
