PDF(Pubmed)
Abstract：
Yeast infections are challenging human and animal medicine due to low rates of detection and the emergence of unknown ecology isolates. The aim of this study was to verify the biochemical identification of yeasts and yeast-like microorganisms obtained from animals comparing the results with chromogenic media and matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF MS). Between January and August 2023, yeast and yeast-like isolates from samples of animals with suspicion of mycosis were identified using Vitek® 2 Compact, Brilliance® Candida Agar and MALDI Biotyper® MSP. A total of 39 cases were included, and 45 isolations were obtained. Cryptococcus neoformans (15.5%, 7/45), Meyerozyma guilliermondii (13.3%, 6/45), Candida parapsilosis (11.1%, 5/45), Candida albicans and Candida tropicalis (8.9%, each one 4/45) were the most identified organisms. There was full agreement with the three identification methods in 71.1% (32/45) of the isolates, disagreement on species in 17.8% (8/45), disagreement on genus and species in 6.7% (3/45) and, in 4.4% (2/45), there was no matched pattern in MALDI-TOF to compare the results. Biochemical methods are a good option in laboratories where proteomics are not available, and chromogenic media enhances diagnostics by detecting mixed infections. Surveillance must be implemented to improve the detection of agents shared between humans and animals.
摘要：
由于低检出率和未知生态分离株的出现，酵母感染对人类和动物医学具有挑战性。这项研究的目的是验证从动物获得的酵母和酵母样微生物的生化鉴定，将结果与显色介质和基质辅助激光解吸/电离飞行时间（MALDI-TOFMS）进行比较。在2023年1月至8月之间，使用Vitek®2Compact鉴定了怀疑有真菌病的动物样本中的酵母和酵母样分离物，华晨®念珠菌琼脂和MALDIBiotyper®MSP。共纳入39例，并获得45个隔离。新生隐球菌(15.5%，7/45),Meyerozymaguilliermondii（13.3%，6/45),近平滑念珠菌(11.1%,5/45),白色念珠菌和热带念珠菌（8.9%，每个4/45）是鉴定最多的生物。71.1%(32/45)的分离株与三种鉴定方法完全一致，对物种的分歧占17.8%(8/45)，6.7%(3/45)的属和种分歧，4.4%(2/45)，MALDI-TOF中没有匹配的模式来比较结果.生化方法在没有蛋白质组学的实验室是一个很好的选择，和显色介质通过检测混合感染增强诊断。必须实施监视，以改善对人类和动物之间共享的病原体的检测。
