Abstract：
Among gastrointestinal nematodes, Haemonchus contortus (Rudolphi) Cobb (order Strongylidae; family Trichostrongylidae) is one of pathogenic and economic importance in domestic and wild ruminants, including the European bison, Bison bonasus Linnaeus (order Cetartiodactyla; family Bovidae); a species on the International Union for Conservation of Nature\'s Red List of Threatened Species. Carabus granulatus Linnaeus (order Coleoptera; family Carabidae) is one of the most prevalent species of ground beetle, inhabiting a wide range of terrestrial ecosystems in Poland. Twenty-six ground beetles of this species inhabiting the Białowieża Primeval Forest in eastern Poland were screened for the presence of DNA of pathogenic gastrointestinal nematodes of ruminants. Extracted DNA was sequenced and compared to reference sequences. In six insects, the presence of H. contortus DNA was detected. The obtained nucleotide sequences were homologous to each other and to the majority of the published DNA sequences of H. contortus isolates. The sequences were also identical to a sequence of H. contortus isolated from European bison in Poland. The study provides the first molecular evidence of the presence of H. contortus DNA in C. granulatus. The finding suggests that ground beetles may play a role in the transmission dynamics of this parasite.
摘要：
在胃肠线虫中，扭曲的血门菌（Rudolphi）Cobb（Strongyialae目；Trichostrongylikyalae科）是家养和野生反刍动物的致病性和经济重要性之一，包括欧洲野牛,BisonbonasusLinnaeus（Cetartiodactyla目；牛科）；国际自然保护联盟濒危物种红色名录中的一个物种。CarabusgranulatusLinnaeus（鞘翅目；Carabidae科）是地面甲虫最普遍的物种之一，居住在波兰广泛的陆地生态系统中。筛选了该物种的26只地面甲虫，这些甲虫居住在波兰东部的Biaswowieāa原始森林中，以检测反刍动物的致病性胃肠道线虫的DNA。对提取的DNA进行测序并与参考序列进行比较。在六种昆虫中，检测到H.contortusDNA的存在。获得的核苷酸序列彼此同源，并且与已发表的H.contortus分离株的大多数DNA序列同源。该序列也与从波兰的欧洲野牛中分离的H.contortus序列相同。该研究提供了在C.granulatus中存在H.contortusDNA的第一个分子证据。该发现表明，地面甲虫可能在这种寄生虫的传播动力学中起作用。
