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Abstract：
Background The pre-malignant tendency of the normal, non-affected portion of the pancreas is not as well explored as the multicentricity documented in pancreatic cancer cases. In order to ascertain the expression of inflammatory markers and Erythroblastic Oncogene B (ErbB2) in the non-affected pancreas in patients with pancreatic cancer, a case-control study was carried out. Materials and methods In patients who underwent pancreatoduodenectomy for pancreatic cancer (PC), pro-inflammatory genes and a tumor marker, erythroblastic oncogene 2 (ErbB2) in the epidermal growth factor receptor family were analyzed in the pancreatic tissue at the cut surface of the normal pancreas using qRT-PCR. Twenty patients diagnosed with Chronic pancreatitis (CP) after Frey\'s surgical procedure were selected, and their pancreatic tissues were analyzed as controls. The HPLC-purified primers were designed using National Center for Biotechnology Information (NCBI) software. The primer\'s specificity was verified for gene expression analysis using the Basic Local Alignment Search Tool (BLAST). The genes under study were normalized using β-actin as the housekeeping gene, and the 2-ddct method was used to compute the fold change compared to the control sample. Results Patients with margin-positive were not included. Pro-inflammatory genes (TNF-α, NF-kβ, and COX-2) had significantly lower foldchange in PC patients compared to the CP group. The CP control group had higher levels of IL-6 gene expression than the PC group. Patients with pancreatic cancer had a considerably higher expression of the ErbB2 gene than patients with CP. Conclusion The upregulated ErbB2 gene in the unaffected pancreatic tissue of pancreatic cancer patients, when compared to controls, indicates that the remaining pancreas may have the capacity to cause cancer. Proto-oncogene may play a role in the pathophysiologic process in patients with pancreatic cancer.
摘要：
背景：正常的癌前倾向，胰腺未受影响的部分没有像胰腺癌病例中记录的多中心那样得到很好的探索。为了确定炎症标志物和红细胞癌基因B（ErbB2）在胰腺癌患者未受影响的胰腺中的表达，进行了一项病例对照研究.材料和方法在接受胰十二指肠切除术治疗胰腺癌（PC）的患者中，促炎基因和肿瘤标志物，使用qRT-PCR在正常胰腺切面的胰腺组织中分析了表皮生长因子受体家族中的红细胞癌基因2（ErbB2）。选择20例Frey手术后诊断为慢性胰腺炎（CP）的患者，和他们的胰腺组织作为对照进行分析。使用国家生物技术信息中心(NCBI)软件设计HPLC纯化的引物。使用基本局部比对搜索工具(BLAST)验证引物的特异性用于基因表达分析。使用β-肌动蛋白作为管家基因对研究中的基因进行归一化，并使用2-ddct方法计算与对照样品相比的倍数变化。结果未纳入切缘阳性患者。促炎基因（TNF-α，NF-kβ,与CP组相比，PC患者中COX-2）的折叠变化显着降低。CP对照组的IL-6基因表达水平高于PC组。胰腺癌患者的ErbB2基因表达明显高于CP患者。结论胰腺癌患者胰腺组织中ErbB2基因表达上调,与对照组相比，表明剩余的胰腺可能具有导致癌症的能力。原癌基因可能在胰腺癌患者的病理生理过程中起作用。
