PDF(Pubmed)
Abstract：
Recent studies have reported on the importance of RBCs in fish responses to viral infections and DNA vaccines. Surface-displaying recombinant bacterins (spinycterins) are a safe and adaptable prototype for viral vaccination of fish and represent an alternative method of aquaculture prophylaxis, since have been reported to enhance fish immune response. We evaluated the innate immune response of rainbow trout (Oncorhynchus mykiss) red blood cells (RBCs), head kidney, and spleen to spinycterins expressing a fragment of the glycoprotein G of viral haemorrhagic septicemia virus (VHSV), one of the most devastating world-wide diseases in farmed salmonids. We first selected an immunorelevant downsized viral fragment of VHSV glycoprotein G (frg16252-450). Then, spinycterins expressing frg16252-450 fused to Nmistic anchor-motif (Nmistic+frg16252-450) were compared to spinycterins expressing frg16252-450 internally without the anchor motif. Nmistic+frg16252-450 spinycterins showed increased attachment to RBCs in vitro and modulated the expression of interferon- and antigen presentation-related genes in RBCs in vitro and in vivo, after intravenous injection. In contrast, the head kidney and spleen of fish injected with frg16252-450, but not Nmistic+frg16252-450, spinycterins demonstrated upregulation of interferon and antigen-presenting genes. Intravenous injection of Nmistic+frg16252-450 spinycterins resulted in a higher innate immune response in RBCs while frg16252-450 spinycterins increased the immune response in head kidney and spleen. Although more studies are required to evaluate the practicality of using spinycterins as fish viral vaccines, these results highlight the important contribution of RBCs to the fish innate immune response to antiviral prophylactics.
摘要：
最近的研究报道了RBC在鱼类对病毒感染和DNA疫苗的反应中的重要性。表面展示重组菌苗（spinycterins）是鱼类病毒疫苗接种的安全且适应性强的原型，代表了水产养殖预防的替代方法。据报道可增强鱼类的免疫反应。我们评估了虹鳟鱼（Oncorhynchusmykiss）红细胞（RBC）的先天免疫反应，头肾,和脾表达病毒性出血性败血病病毒（VHSV）的糖蛋白G片段的spinycterins，养殖鲑鱼中最具破坏性的世界性疾病之一。我们首先选择了VHSV糖蛋白G的免疫相关的缩小病毒片段(frg16252-450)。然后，将表达frg16252-450融合到Nvical锚定基序（Nvicalfrg16252-450）的spinycterin与内部表达frg16252-450而没有锚定基序的spinycterin进行比较。Nvicalfrg16252-450棘突蛋白在体外显示出对红细胞的附着增加，并在体外和体内调节红细胞中干扰素和抗原呈递相关基因的表达，静脉注射后。相比之下,注射frg16252-450的鱼的头肾和脾脏，但未注射Ngvicalfrg16252-450，spinycelins显示干扰素和抗原呈递基因上调。静脉内注射Ngomyfrg16252-450刺蝶呤导致红细胞中更高的先天性免疫反应，而frg16252-450刺蝶呤增加了头肾和脾脏中的免疫反应。尽管需要更多的研究来评估使用刺蝶呤作为鱼类病毒疫苗的实用性，这些结果突出了红细胞对鱼类对抗病毒预防的先天免疫应答的重要贡献。
