PDF(Pubmed)
Abstract：
Procollagen c-protease enhancer protein (PCOLCE) performs an essential action in improving the recreation of procollagen c-protease and promoting the reconstruction of extracellular matrix. High PCOLCE expression was associated with a negative prognosis of stomach cancer, ovarian cancer, and osteosarcoma. The goal of this work is to investigate the function of PCOLCE in glioma. Multiple bioinformatics techniques have been employed to investigate the roles of PCOLCE in glioma, consisting of the correlation between PCOLCE and prognosis, immune checkpoints, immune cell infiltrates, and tumor microenvironment (TME). The gene ontology (GO) annotations and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were used to assess the potential function of PCOLCE in glioma. PCOLCE was found to be increased in glioma. We revealed that PCOLCE was a potential prognostic factor and related to tumor grade. Up-regulated PCOLCE was related to poor prognosis in lower-grade glioma (LGG), glioblastoma multiforme (GBM), and recurrent glioma. PCOLCE was correlated with immune cell infiltration, particularly B cells, CD4+ T cells, macrophages, neutrophils, and dendritic cells (DCs) in LGG, and DCs infiltration in GBM. PCOLCE was co-expressed with many genes related to the immune and the immune checkpoint. In addition, glioma patients with low expression of PCOLCE had a higher response to the immunological checkpoint blockade (ICB). Additionally, PCOLCE may exert its roles via several immune-related biological processes or pathways, such as leukocyte migration, activation of T cells, adaptive immune response, neutrophil-mediated immunity, NF-κB, and TNF signaling pathways. In conclusion, PCOLCE may be a new immune-related gene and regulate tumor development through immunological pathways.
摘要：
前胶原c-蛋白酶增强蛋白（PCLCE）在改善前胶原c-蛋白酶的再生和促进细胞外基质的重建方面具有重要作用。PCLCE高表达与胃癌的阴性预后相关，卵巢癌,和骨肉瘤.这项工作的目的是研究PCLCE在神经胶质瘤中的功能。多种生物信息学技术已被用于研究PCLCE在神经胶质瘤中的作用，由PCLCE和预后之间的相关性组成，免疫检查点,免疫细胞浸润，和肿瘤微环境（TME）。基因本体论（GO）注释和京都基因和基因组百科全书（KEGG）分析用于评估PCLCE在神经胶质瘤中的潜在功能。发现PCLCE在神经胶质瘤中增加。我们发现PCLCE是一个潜在的预后因素，与肿瘤分级有关。上调PCLCE与低级别胶质瘤（LGG）的不良预后相关，多形性胶质母细胞瘤（GBM），和复发性神经胶质瘤.PCLCE与免疫细胞浸润相关，特别是B细胞，CD4+T细胞,巨噬细胞，中性粒细胞，和LGG中的树突状细胞（DC），GBM中的DCs浸润。PCLCE与许多与免疫和免疫检查点相关的基因共表达。此外,PCLCE低表达的胶质瘤患者对免疫检查点阻断(ICB)的反应较高.此外,PCLCE可能通过几种免疫相关的生物学过程或途径发挥作用，如白细胞迁移，激活T细胞，适应性免疫反应，中性粒细胞介导的免疫，NF-κB,和TNF信号通路。总之,PCLCE可能是一个新的免疫相关基因，通过免疫学途径调控肿瘤的发展。
