PDF(Pubmed)
Abstract：
UNASSIGNED: Craniofacial growth and development are more than a scientific curiosity; it is of tremendous interest to clinicians. Insights into the genetic etiology of cleft lip and palate development are essential for improving diagnosis and treatment planning. The purpose of this systematic review was to utilize a zebrafish model to highlight the role of the IRF6 gene in cleft lip and palate development in humans.
UNASSIGNED: This review adhered to the guidelines outlined in the PRISMA statement. Nine studies were included in the analysis.
UNASSIGNED: This study used major scientific databases such as MEDLINE, EMBASE, Web of Science, and the Zebrafish Information Network and yielded 1275 articles. Two reviewers performed the screening using COVIDENCE™ independently, and a third reviewer resolved any conflicts.
UNASSIGNED: After applying the inclusion and exclusion criteria and screening, nine studies were included in the analysis. The Systematic Review Center for Laboratory Animal Experimentation\'s (SYRCLE\'s) risk-of-bias tool was used to assess the quality of the included studies.
UNASSIGNED: The main outcome supports the role of the IRF6 gene in zebrafish periderm development and embryogenesis, and IRF6 variations result in cleft lip and palate development. The overall SYRCLE risk of bias was low-medium.
UNASSIGNED: In conclusion, this review indicated the critical role of the IRF6 gene and its downstream genes (GRHL3, KLF17, and ESRP1/2) in the development of cleft lip and palate in zebrafish models. Genetic mutation zebrafish models provide a high level of insights into zebrafish craniofacial development.
UNASSIGNED: this review provides a productive avenue for understanding the powerful and conserved zebrafish model for investigating the pathogenesis of human cleft lip and palate.
UNASSIGNED: This review adhered to the guidelines outlined in the PRISMA statement. Nine studies were included in the analysis.
UNASSIGNED: This study used major scientific databases such as MEDLINE, EMBASE, Web of Science, and the Zebrafish Information Network and yielded 1275 articles. Two reviewers performed the screening using COVIDENCE™ independently, and a third reviewer resolved any conflicts.
UNASSIGNED: After applying the inclusion and exclusion criteria and screening, nine studies were included in the analysis. The Systematic Review Center for Laboratory Animal Experimentation\'s (SYRCLE\'s) risk-of-bias tool was used to assess the quality of the included studies.
UNASSIGNED: The main outcome supports the role of the IRF6 gene in zebrafish periderm development and embryogenesis, and IRF6 variations result in cleft lip and palate development. The overall SYRCLE risk of bias was low-medium.
UNASSIGNED: In conclusion, this review indicated the critical role of the IRF6 gene and its downstream genes (GRHL3, KLF17, and ESRP1/2) in the development of cleft lip and palate in zebrafish models. Genetic mutation zebrafish models provide a high level of insights into zebrafish craniofacial development.
UNASSIGNED: this review provides a productive avenue for understanding the powerful and conserved zebrafish model for investigating the pathogenesis of human cleft lip and palate.
摘要：
■颅面生长和发育不仅仅是科学的好奇心；临床医生对此非常感兴趣。对唇裂和腭裂发育的遗传病因的见解对于改善诊断和治疗计划至关重要。本系统综述的目的是利用斑马鱼模型来强调IRF6基因在人类唇腭裂发育中的作用。
■本次审查遵循了PRISMA声明中概述的指导方针。九项研究纳入分析。
■这项研究使用了主要的科学数据库，例如MEDLINE，EMBASE,WebofScience,和斑马鱼信息网络，并产生了1275篇文章。两名审核员独立使用COVIDENCE™进行筛查，第三个审稿人解决了任何冲突。
■应用纳入和排除标准和筛选后，9项研究纳入分析.实验动物实验系统评价中心（SYRCLE）的偏倚风险工具用于评估纳入研究的质量。
■主要结果支持IRF6基因在斑马鱼周皮发育和胚胎发生中的作用，和IRF6变异导致唇腭裂发育。总体SYRCLE偏倚风险为中低。
■总而言之，这篇综述指出了IRF6基因及其下游基因（GRHL3，KLF17和ESRP1/2）在斑马鱼模型中唇腭裂发育中的关键作用。基因突变斑马鱼模型提供了对斑马鱼颅面发育的高度见解。
■这篇综述为理解强大而保守的斑马鱼模型提供了一条富有成效的途径，用于研究人类唇腭裂的发病机理。
■本次审查遵循了PRISMA声明中概述的指导方针。九项研究纳入分析。
■这项研究使用了主要的科学数据库，例如MEDLINE，EMBASE,WebofScience,和斑马鱼信息网络，并产生了1275篇文章。两名审核员独立使用COVIDENCE™进行筛查，第三个审稿人解决了任何冲突。
■应用纳入和排除标准和筛选后，9项研究纳入分析.实验动物实验系统评价中心（SYRCLE）的偏倚风险工具用于评估纳入研究的质量。
■主要结果支持IRF6基因在斑马鱼周皮发育和胚胎发生中的作用，和IRF6变异导致唇腭裂发育。总体SYRCLE偏倚风险为中低。
■总而言之，这篇综述指出了IRF6基因及其下游基因（GRHL3，KLF17和ESRP1/2）在斑马鱼模型中唇腭裂发育中的关键作用。基因突变斑马鱼模型提供了对斑马鱼颅面发育的高度见解。
■这篇综述为理解强大而保守的斑马鱼模型提供了一条富有成效的途径，用于研究人类唇腭裂的发病机理。
