PDF(Pubmed)
Abstract：
Clinical studies indicate antibiotic bone cement with propeller flaps improves diabetic foot wound repair and reduces amputation rates, but the molecular mechanisms, particularly key proteins\' role remain largely unexplored. This study assessed the efficacy of antibiotic bone cement for treating diabetic foot wounds, focusing on molecular impact on ROCK1. Sixty patients were randomized into experimental (EXP, n = 40) and control (CON, n = 20) groups, treated with antibiotic bone cement and negative pressure. Wound healing rate, amputation rate, wound secretion culture and C-reactive protein (CRP) changes, were monitored. Comprehensive molecular investigations were conducted and animal experiments were performed to further validate the findings. Statistical methods were employed to verify significant differences between the groups and treatment outcomes. The EXP group showed significant improvements in wound healing ( χ 2 $$ {\\chi}^2 $$ = 11.265, p = 0.004) and reduced amputation rates. Elevated levels of ROCK1, fibroblasts and VGF were observed in the trauma tissue post-treatment in the experimental group compared to pre-treatment and the control group (all p < 0.05). Improved trauma secretion culture and CRP were also noted in the EXP group (all p < 0.05). The study suggests that antibiotic bone cement enhances diabetic foot wound healing, possibly via upregulation of ROCK1. Further research is needed to elucidate the underlying molecular mechanisms and broader clinical implications.
摘要：
临床研究表明，带螺旋桨皮瓣的抗生素骨水泥可改善糖尿病足伤口修复并降低截肢率，但是分子机制，特别是关键蛋白质的作用在很大程度上仍未被探索。这项研究评估了抗生素骨水泥治疗糖尿病足伤口的疗效，关注分子对ROCK1的影响。60例患者被随机分为实验组(EXP，n=40)和控制(CON，n=20)组，用抗生素骨水泥和负压治疗。伤口愈合率,截肢率,伤口分泌物培养和C反应蛋白（CRP）的变化，被监控。进行了全面的分子研究并进行了动物实验以进一步验证发现。采用统计学方法来验证组间和治疗结果之间的显著差异。EXP组伤口愈合显著改善(χ2$${\\chi}^2$$=11.265,p=0.004),截肢率降低。实验组治疗后创伤组织中ROCK1、成纤维细胞和VGF水平均高于治疗前和对照组(均P<0.05)。在EXP组中还观察到改善的创伤分泌培养和CRP(均p<0.05)。研究表明，抗生素骨水泥可增强糖尿病足伤口的愈合，可能通过上调ROCK1。需要进一步的研究来阐明潜在的分子机制和更广泛的临床意义。
