Abstract：
OBJECTIVE: To compare 2 point-of-care lateral flow assays (LFAs) with immunodiffusion (ID) IgG results for anti-coccidioidal antibody detection in dogs with coccidioidomycosis. A further aim was to compare the quantifiable output of 1 of the LFAs to ID antibody titers.
METHODS: Serum banked from 73 client-owned dogs diagnosed with pulmonary or disseminated coccidioidomycosis.
METHODS: ID was used to determine antibody presence and titer against a coccidioidal antigen preparation. All sera were subsequently tested on an LFA based on recombinant chitinase 1 (CTS1) and the commercially available sōna LFA. LFA results were analyzed and compared to ID IgG results and clinical diagnosis.
RESULTS: All assays showed similar sensitivities in detecting anti-coccidioidal antibodies (83.6% to 89.0%). When compared with ID IgG, the CTS1 LFA had a positive percent agreement of 100%, while the sōna LFA had a positive percent agreement of 91.4%. Since the CTS1 LFA is semiquantitative, we were able to compare test line densities with ID titers and found a strong correlation between the 2 assays (Spearman ρ = 0.82).
CONCLUSIONS: This is the first side-by-side evaluation of a commercially available LFA (sōna) and a newer more rapid anti-CTS1 antibody LFA using serum from dogs with coccidioidomycosis. Both LFAs tested have similar sensitivity to ID IgG results. The CTS1 LFA can be read after 10 minutes and is semiquantitative, while the sōna LFA is read after 30 minutes, and the results are subject to interpretation. Accurate and fast detection of anti-coccidioidal antibodies allows clinicians to initiate appropriate treatment without diagnostic delay.
METHODS: Serum banked from 73 client-owned dogs diagnosed with pulmonary or disseminated coccidioidomycosis.
METHODS: ID was used to determine antibody presence and titer against a coccidioidal antigen preparation. All sera were subsequently tested on an LFA based on recombinant chitinase 1 (CTS1) and the commercially available sōna LFA. LFA results were analyzed and compared to ID IgG results and clinical diagnosis.
RESULTS: All assays showed similar sensitivities in detecting anti-coccidioidal antibodies (83.6% to 89.0%). When compared with ID IgG, the CTS1 LFA had a positive percent agreement of 100%, while the sōna LFA had a positive percent agreement of 91.4%. Since the CTS1 LFA is semiquantitative, we were able to compare test line densities with ID titers and found a strong correlation between the 2 assays (Spearman ρ = 0.82).
CONCLUSIONS: This is the first side-by-side evaluation of a commercially available LFA (sōna) and a newer more rapid anti-CTS1 antibody LFA using serum from dogs with coccidioidomycosis. Both LFAs tested have similar sensitivity to ID IgG results. The CTS1 LFA can be read after 10 minutes and is semiquantitative, while the sōna LFA is read after 30 minutes, and the results are subject to interpretation. Accurate and fast detection of anti-coccidioidal antibodies allows clinicians to initiate appropriate treatment without diagnostic delay.
摘要：
目的：比较2点护理侧流测定（LFAs）和免疫扩散（ID）IgG结果，以检测球虫菌病犬的抗球虫抗体。另一个目的是比较1个LFA的可量化输出与ID抗体滴度。
方法：从73只被诊断为肺部或播散型球孢子菌病的客户拥有的狗中收集血清。
方法：ID用于确定针对球虫抗原制剂的抗体存在和滴度。随后在基于重组几丁质酶1（CTS1）和市售s_naLFA的LFA上测试所有血清。分析LFA结果并与IDIgG结果和临床诊断进行比较。
结果：所有测定在检测抗球虫抗体方面均显示相似的敏感性（83.6％至89.0％）。与IDIgG相比，CTS1LFA具有100%的正百分比一致性，而sōnaLFA的正百分比一致性为91.4%。由于CTS1LFA是半定量的，我们能够将测试线密度与ID滴度进行比较，并发现2种测定之间存在很强的相关性（Spearmanρ=0.82）。
结论：这是对市售LFA（sōna）和较新的更快速的抗CTS1抗体LFA进行的首次并排评估，使用来自球虫菌病犬的血清。测试的两种LFA对IDIgG结果具有相似的敏感性。CTS1LFA可以在10分钟后读取，并且是半定量的，而在30分钟后阅读sōnaLFA，结果有待解释。抗球虫抗体的准确和快速检测允许临床医生在没有诊断延迟的情况下开始适当的治疗。
方法：从73只被诊断为肺部或播散型球孢子菌病的客户拥有的狗中收集血清。
方法：ID用于确定针对球虫抗原制剂的抗体存在和滴度。随后在基于重组几丁质酶1（CTS1）和市售s_naLFA的LFA上测试所有血清。分析LFA结果并与IDIgG结果和临床诊断进行比较。
结果：所有测定在检测抗球虫抗体方面均显示相似的敏感性（83.6％至89.0％）。与IDIgG相比，CTS1LFA具有100%的正百分比一致性，而sōnaLFA的正百分比一致性为91.4%。由于CTS1LFA是半定量的，我们能够将测试线密度与ID滴度进行比较，并发现2种测定之间存在很强的相关性（Spearmanρ=0.82）。
结论：这是对市售LFA（sōna）和较新的更快速的抗CTS1抗体LFA进行的首次并排评估，使用来自球虫菌病犬的血清。测试的两种LFA对IDIgG结果具有相似的敏感性。CTS1LFA可以在10分钟后读取，并且是半定量的，而在30分钟后阅读sōnaLFA，结果有待解释。抗球虫抗体的准确和快速检测允许临床医生在没有诊断延迟的情况下开始适当的治疗。
